Immobilization of pancreatic porcine lipase in calcium and barium alginate comparative studies of their kinetic properties and screening the polymer for suitability

Abstract

Purpose: The objective of the study was to select a suitable polymer for immobilization of pancreatic porcine lipase by entrapment method and to evaluate the kinetic properties of immobilized enzyme with free enzyme. Method:. Enzyme was immobilized by entrapment method using calcium alginate and barium alginate polymers. Lipase activity was determined by titrimetric assay procedure. Km and Vmax were determined with Michaelis Menton Equation. Result: The present work demonstrated that calcium alginate beads loaded with enzyme is more stable at high temperature and has excellent Vmax and Km in comparison with barium alginate beads.. Compare to immobilized enzyme free enzyme could not withstand high temperature. Conclusion: Sodium alginate polymer is an excellent carrier for entrapment of pancreatic porcine lipase.