PRODUCTION, PURIFICATION AND CHARACTERIZATION OF AN ANTIBACTERIAL COMPOUND FROM STREPTOMYCES GRISEUS TBG19NRA1 ISOLATED FROM THE FOREST SOILS OF THE WESTERN GHATS OF KERALA, INDIA

The Journal of Microbiology, Biotechnology and Food Sciences Pub Date : 2021-04-07 DOI:10.15414/JMBFS.590

S. Sugathan, Gayathri Valsala, Jeeshma Nambidi Parambath, K. Menon, Rameshkumar Koranappallil Bahuleyan

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引用次数: 1

Abstract

A new actinomycete strain designated TBG19NRA1, exhibiting antimicrobial activities against Gram-positive and Gram-negative bacteria, was isolated from forest soil of Neyyar Wild Life Sanctuary, Thiruvananthapuram, Kerala. Cultural characteristics strongly suggested that this isolate belongs to the genus Streptomyces. Polyphasic taxonomic studies including phylogenetic analysis based on 16S rDNA sequence suggests that the isolate is a strain of S. griseus. Studies on the effect of different nutritional compounds on antibiotic activity revealed that the highest antibacterial activity was obtained when 1% starch (w/v) was used as the sole carbon source along with mineral trace elements. Extraction and purification of TBG19NRA1 culture supernatant led to the isolation of a pure molecule with good antibacterial activity. The compound has been identified as the cyclic polyether lasalocid A on the basis IR, MS and 13C NMR data interpretation and comparison with reference data from literature.

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从印度喀拉拉邦西部高止山脉森林土壤中分离的灰色链霉菌tbg19nra1抗菌化合物的制备、纯化和鉴定

从印度喀拉拉邦蒂鲁凡anthapuram Neyyar野生动物保护区森林土壤中分离到一株对革兰氏阳性菌和革兰氏阴性菌均有抑菌活性的放线菌TBG19NRA1。培养特征强烈表明该分离物属于链霉菌属。多相分类研究包括基于16S rDNA序列的系统发育分析表明，该分离物是一株金黄色葡萄球菌。对不同营养物对抗菌活性的影响进行了研究，结果表明，以1%淀粉(w/v)为唯一碳源，外加矿物质微量元素时，抗菌活性最高。对TBG19NRA1培养上清进行提取纯化，分离出具有良好抗菌活性的纯分子。通过IR、MS和13C NMR数据的解释以及与文献参考数据的比较，确定该化合物为环聚醚lasalod A。

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The Journal of Microbiology, Biotechnology and Food Sciences

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