The impact of TP53 Arg72Pro and R249S genetic variations on IVF-ET outcome

Abstract

In-vitro fertilization-embryo transfer (IVF-ET) can be mentioned as one of the most important methods that various environmental and genetic factors are involved in its success or failure. Present research aims at studying the importance of Pro72Arg and Arg249Ser of TP53 gene in success of IVF-ET. One hundred and fifty infertile women with unsuccessful IVF-ET history and 210 women with successful IVF-ET history were studied using polymerase chain reaction– restriction fragment length polymorphism (PCR–RFLP) and amplification refractory mutation system (ARMS-PCR). The P53 codon 249 Ser allele was found to be over-represented in patients (p=0.0001; Odds Ratio=3.407; 95% CI; 1.81-6.38). In this study, the distribution of the genotype at codon 72 in the unsuccessful IVF women was not different from the controls (p=0.5). In conclusion, despite the P53 codon 249 Ser allele appearing to be associated with a trend of increased risk of IVF failure, P53 Pro72Arg gene polymorphism might not be a useful marker for prediction of IVF-ET outcome. However, in order to confirm the results, the studies are recommended to be conducted in larger populations.