Microsomes and enzymes immobilized on grafted polyethylenes

Abstract

Immobilization of microsomal fractions, proteolytic enzymes and urease on polyethylene-grafted poly(acrylic acid), poly(allyl alcohol) and poly(allylamine) was investigated. Immobilization of microsomal fractions on polyethylene-grafted poly(allyl alcohol) and poly(acrylic acid) preserved 108 and 31.5% of aniline hydroxylase activity and 125 and 109% of N,N-dimethylaniline demethylase activity, respectively. An increased pH, temperature, and storage stability of the immobilized microsomes was observed. Column operation was performed as well. Positive results were also obtained in the immobilization of urease and Bacillus mesentericus protease.