Expression analysis of miR-2005 and its target genes in Apostichopus japonicus by Vibrio splendidus challenged

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2019-07-04 DOI:10.25431/1824-307X/ISJ.V0I0.105-112

Xiaoxu Zhou, X. Chang, Yaoyao Zhan, Xiuli Wang, Kai Lin

{"title":"Expression analysis of miR-2005 and its target genes in Apostichopus japonicus by Vibrio splendidus challenged","authors":"Xiaoxu Zhou, X. Chang, Yaoyao Zhan, Xiuli Wang, Kai Lin","doi":"10.25431/1824-307X/ISJ.V0I0.105-112","DOIUrl":null,"url":null,"abstract":"MicroRNAs (miRNAs) are important effectors in mediating host-pathogen interaction. MiR-2005 is observed to be involved in immune response processes in Apostichopus japonicus. In the present study, the putative target genes of miR-2005 in A. japonicus coelomocytes were predicted by bioinformatics analysis of transcriptome database and PCR approaches. A total of 506 potential targets were screened, and 187 targets were annotated. Several immune-related target genes were identified in this study, such as SLI3, CFHR5, FGL, A2ML, and Rab9al. The expression patterns of miR-2005 and its potential targets were validated by quantitative real-time PCR in Vibrio splendidus challenged A. japonicus. For further characterization, an overexpression experiment of miR-2005 at cellular levels was applied. Accordingly, significant negative correlation expression profiles were detected between miR-2005 and two candidates targets, suggesting that SLI3 and CHRP5 showed high possibility to be the targets of miR-2005 in A. japonicus. Altogether, this study will enhance our understanding in the context of miR-2005 modulating the interaction of A. japonicus after being challenged by V. splendidus.","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2019-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.25431/1824-307X/ISJ.V0I0.105-112","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 1

Abstract

MicroRNAs (miRNAs) are important effectors in mediating host-pathogen interaction. MiR-2005 is observed to be involved in immune response processes in Apostichopus japonicus. In the present study, the putative target genes of miR-2005 in A. japonicus coelomocytes were predicted by bioinformatics analysis of transcriptome database and PCR approaches. A total of 506 potential targets were screened, and 187 targets were annotated. Several immune-related target genes were identified in this study, such as SLI3, CFHR5, FGL, A2ML, and Rab9al. The expression patterns of miR-2005 and its potential targets were validated by quantitative real-time PCR in Vibrio splendidus challenged A. japonicus. For further characterization, an overexpression experiment of miR-2005 at cellular levels was applied. Accordingly, significant negative correlation expression profiles were detected between miR-2005 and two candidates targets, suggesting that SLI3 and CHRP5 showed high possibility to be the targets of miR-2005 in A. japonicus. Altogether, this study will enhance our understanding in the context of miR-2005 modulating the interaction of A. japonicus after being challenged by V. splendidus.

查看原文本刊更多论文

脾弧菌攻毒后miR-2005及其靶基因在日本刺参中的表达分析

MicroRNAs (miRNAs)是介导宿主-病原体相互作用的重要效应物。MiR-2005被观察到参与Apostichopus japonicus的免疫应答过程。本研究通过转录组数据库的生物信息学分析和PCR方法预测了日本刺参体腔细胞中miR-2005的可能靶基因。共筛选了506个潜在靶点，并对187个靶点进行了注释。本研究确定了几个免疫相关靶基因，如SLI3、CFHR5、FGL、A2ML和Rab9al。通过实时荧光定量PCR验证了miR-2005及其潜在靶点在脾弧菌侵染的日本刺参中的表达模式。为了进一步表征，我们在细胞水平上进行了miR-2005过表达实验。因此，miR-2005与两个候选靶点之间存在显著的负相关表达谱，表明SLI3和CHRP5极有可能成为粳稻中miR-2005的靶点。总之，本研究将加深我们对miR-2005调控日本稻蛾在被黄弧菌攻毒后相互作用的理解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.