Expression analysis of miR-2005 and its target genes in Apostichopus japonicus by Vibrio splendidus challenged

IF 1.2 4区农林科学 Q4 IMMUNOLOGY

ISJ-Invertebrate Survival Journal Pub Date : 2019-07-04 DOI:10.25431/1824-307X/ISJ.V0I0.105-112

Xiaoxu Zhou, X. Chang, Yaoyao Zhan, Xiuli Wang, Kai Lin

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引用次数: 1

Abstract

MicroRNAs (miRNAs) are important effectors in mediating host-pathogen interaction. MiR-2005 is observed to be involved in immune response processes in Apostichopus japonicus. In the present study, the putative target genes of miR-2005 in A. japonicus coelomocytes were predicted by bioinformatics analysis of transcriptome database and PCR approaches. A total of 506 potential targets were screened, and 187 targets were annotated. Several immune-related target genes were identified in this study, such as SLI3, CFHR5, FGL, A2ML, and Rab9al. The expression patterns of miR-2005 and its potential targets were validated by quantitative real-time PCR in Vibrio splendidus challenged A. japonicus. For further characterization, an overexpression experiment of miR-2005 at cellular levels was applied. Accordingly, significant negative correlation expression profiles were detected between miR-2005 and two candidates targets, suggesting that SLI3 and CHRP5 showed high possibility to be the targets of miR-2005 in A. japonicus. Altogether, this study will enhance our understanding in the context of miR-2005 modulating the interaction of A. japonicus after being challenged by V. splendidus.

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脾弧菌攻毒后miR-2005及其靶基因在日本刺参中的表达分析

MicroRNAs (miRNAs)是介导宿主-病原体相互作用的重要效应物。MiR-2005被观察到参与Apostichopus japonicus的免疫应答过程。本研究通过转录组数据库的生物信息学分析和PCR方法预测了日本刺参体腔细胞中miR-2005的可能靶基因。共筛选了506个潜在靶点，并对187个靶点进行了注释。本研究确定了几个免疫相关靶基因，如SLI3、CFHR5、FGL、A2ML和Rab9al。通过实时荧光定量PCR验证了miR-2005及其潜在靶点在脾弧菌侵染的日本刺参中的表达模式。为了进一步表征，我们在细胞水平上进行了miR-2005过表达实验。因此，miR-2005与两个候选靶点之间存在显著的负相关表达谱，表明SLI3和CHRP5极有可能成为粳稻中miR-2005的靶点。总之，本研究将加深我们对miR-2005调控日本稻蛾在被黄弧菌攻毒后相互作用的理解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ISJ-Invertebrate Survival Journal IMMUNOLOGY-ZOOLOGY

CiteScore

2.10

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Invertebrate Survival Journal (ISJ) is an international and open access journal devoted to prompt and innovative studies on the basic defense mechanisms in invertebrates, in particular with a view to identifying biotechnologies able to act against derived diseases and related economic damage. Contributions will be mainly in the form of Letters to the Editor, Visions and Perspectives, Short Communications, Technical Reports, Research Reports, Review, Minireview and Reports of Meetings. Letters to the Editor can be commentaries or perspectives on invertebrate defence mechanisms or replies to the data published in ISJ.