Mass spectrometry: a technique of many faces

IF 7.2 2区生物学 Q1 BIOPHYSICS

Quarterly Reviews of Biophysics Pub Date : 2016-11-28 DOI:10.1017/S0033583516000160

Maya A. Olshina, M. Sharon

引用次数: 11

Abstract

Abstract Protein complexes form the critical foundation for a wide range of biological process, however understanding the intricate details of their activities is often challenging. In this review we describe how mass spectrometry (MS) plays a key role in the analysis of protein assemblies and the cellular pathways which they are involved in. Specifically, we discuss how the versatility of mass spectrometric approaches provides unprecedented information on multiple levels. We demonstrate this on the ubiquitin-proteasome proteolytic pathway, a process that is responsible for protein turnover. We follow the various steps of this degradation route and illustrate the different MS workflows that were applied for elucidating molecular information. Overall, this review aims to stimulate the integrated use of multiple mass spectrometry approaches for analyzing complex biological systems.

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质谱分析:一项多方面的技术

蛋白质复合物是多种生物过程的重要基础，然而，了解其活动的复杂细节往往具有挑战性。在这篇综述中，我们描述了质谱(MS)如何在蛋白质组装及其参与的细胞途径的分析中发挥关键作用。具体来说，我们讨论了质谱方法的多功能性如何在多个层面上提供前所未有的信息。我们在泛素-蛋白酶体蛋白水解途径上证明了这一点，这是一个负责蛋白质周转的过程。我们遵循这种降解途径的各个步骤，并说明用于阐明分子信息的不同MS工作流程。总之，这篇综述旨在促进多种质谱方法的综合使用，以分析复杂的生物系统。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Quarterly Reviews of Biophysics 生物-生物物理

CiteScore

12.90

自引率

1.60%

发文量

期刊介绍： Quarterly Reviews of Biophysics covers the field of experimental and computational biophysics. Experimental biophysics span across different physics-based measurements such as optical microscopy, super-resolution imaging, electron microscopy, X-ray and neutron diffraction, spectroscopy, calorimetry, thermodynamics and their integrated uses. Computational biophysics includes theory, simulations, bioinformatics and system analysis. These biophysical methodologies are used to discover the structure, function and physiology of biological systems in varying complexities from cells, organelles, membranes, protein-nucleic acid complexes, molecular machines to molecules. The majority of reviews published are invited from authors who have made significant contributions to the field, who give critical, readable and sometimes controversial accounts of recent progress and problems in their specialty. The journal has long-standing, worldwide reputation, demonstrated by its high ranking in the ISI Science Citation Index, as a forum for general and specialized communication between biophysicists working in different areas. Thematic issues are occasionally published.