Evaluation of Gelidiella acerosa, the red algae inhabiting South Indian coastal area for antioxidant and metal chelating potential

Biomedicine & Preventive Nutrition Pub Date : 2013-10-01 DOI:10.1016/j.bionut.2013.03.007

N. Suganthy, S. Arif Nisha, S. Karutha Pandian, K. Pandima Devi

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引用次数: 19

Abstract

In vitro antioxidant potential and metal chelating activity of various solvent fractions of Gelidiella acerosa were evaluated by different antioxidant assays, like ferric reducing antioxidative power (FRAP) assay and scavenging activities for hydrogen peroxide, hydroxyl radical and nitric oxide. Among all the fractions, benzene showed the highest 1,1-diphenyl,2-picrylhydrazyl (DPPH) radical, nitric oxide and hydrogen peroxide scavenging activity when compared to standard BHT with IC₅₀ values of 306.50 ± 3.04, 328.79 ± 14.25 and 275.43 ± 72.99 μg/mL, respectively. Total antioxidative capacity and reducing power was found to be significantly higher in the dichloromethane fraction. Highest ferrous ion chelating activity was observed in ethyl acetate and benzene fractions. Dimethylsulfoxide fraction exerted the highest hydroxyl radical scavenging effect. Moreover, benzene and dichloromethane fraction showed the highest total polyphenolic content of 18.86 ± 0.27 and 17.69 ± 0.353 μg/mg of dry extract, respectively. Preliminary cytotoxic studies suggest that benzene and dichloromethane fraction has no cytotoxic effect, hence, they can be used as effective antioxidant for treating reactive oxygen species (ROS) mediated diseases.

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南印度沿海红藻尖胶藻的抗氧化和金属螯合能力评价

通过铁还原抗氧化能力(FRAP)、过氧化氢、羟基自由基和一氧化氮的清除能力等抗氧化指标，评价了不同溶剂组分的体外抗氧化能力和金属螯合活性。其中，苯与标准BHT相比，对1,1-二苯基、2-苦味肼基(DPPH)自由基、一氧化氮和过氧化氢的清除活性最高，IC50值分别为306.50±3.04、328.79±14.25和275.43±72.99 μg/mL。总抗氧化能力和还原能力在二氯甲烷馏分中显著提高。乙酸乙酯和苯馏分中铁离子螯合活性最高。二甲亚砜组分对羟基自由基的清除作用最强。其中，苯和二氯甲烷组分的总多酚含量最高，分别为18.86±0.27和17.69±0.353 μg/mg。初步的细胞毒性研究表明，苯和二氯甲烷组分没有细胞毒性作用，因此，它们可以作为有效的抗氧化剂治疗活性氧(ROS)介导的疾病。

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Biomedicine & Preventive Nutrition

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