Identification and functional analysis of foxl2 and nodal in sea cucumber, Apostichopus japonicus

IF 1 4区生物学 Q4 DEVELOPMENTAL BIOLOGY

Gene Expression Patterns Pub Date : 2022-06-01 DOI:10.1016/j.gep.2022.119245

Juan-Juan Sun , Zhi-Hui Sun , Jin-Liang Wei, Jun Ding, Jian Song, Ya-Qing Chang

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引用次数: 4

Abstract

Sea cucumber (Apostichopus japonicus) is an important mariculture species in China. To date, the mechanisms of sex determination and differentiation in sea cucumber remain unclear. Identifying sex-specific molecular markers is an effective method for revealing the genetic basis of sex determination and sex differentiation. In this study, foxl2 and nodal homologous genes were identified in A. japonicus. Foxl2 exhibited dynamic and sexually dimorphic expression patterns in the gonads, with prominent expression in the ovaries and minimal expression in the testis according to real-time quantitative PCR (RT-qPCR) study. As nodal was specifically expressed in the ovary, it could serve as an ovary-specific marker in sea cucumber. Additionally, knockdown of foxl2 or nodal using RNA interference (RNAi) led to the down-regulation of piwi, germ cell-less, and dmrt1, suggesting that foxl2 and nodal may play important roles in gonad maintenance of sea cucumber. Overall, this study adds to our understanding of the roles of foxl2 and nodal in the gonadal development of A. japonicus, which provides further insight into the mechanisms of sea cucumber sex determination and differentiation.

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Apostichopus japonicus海参foxl2和nodal的鉴定及功能分析

海参(Apostichopus japonicus)是中国重要的海水养殖品种。迄今为止，海参性别决定和分化的机制尚不清楚。鉴定性别特异性分子标记是揭示性别决定和性别分化的遗传基础的有效方法。本研究在日本刺参中鉴定出foxl2和节点同源基因。实时定量PCR (RT-qPCR)结果显示，Foxl2在性腺中表现出动态和两性二态表达模式，在卵巢中表达突出，在睾丸中表达较少。nodal在海参卵巢中特异表达，可作为海参卵巢特异性标志物。此外，通过RNA干扰(RNAi)敲低foxl2或nodal导致piwi、germ -less和dmrt1的下调，提示foxl2和nodal可能在海参性腺维持中发挥重要作用。总之，本研究增加了我们对foxl2和nodal在A. japonicus性腺发育中的作用的认识，为进一步了解海参性别决定和分化的机制提供了新的思路。

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来源期刊

Gene Expression Patterns 生物-发育生物学

CiteScore

2.30

自引率

0.00%

发文量

审稿时长

35 days

期刊介绍： Gene Expression Patterns is devoted to the rapid publication of high quality studies of gene expression in development. Studies using cell culture are also suitable if clearly relevant to development, e.g., analysis of key regulatory genes or of gene sets in the maintenance or differentiation of stem cells. Key areas of interest include: -In-situ studies such as expression patterns of important or interesting genes at all levels, including transcription and protein expression -Temporal studies of large gene sets during development -Transgenic studies to study cell lineage in tissue formation