In-Vitro Regeneration of Interspecific F1 Hybrid (Eucalyptus citriodora and Eucalyptus torelliana) of Eucalyptus

Abstract

Tissue culture technique was standardized for in vitro shoot multiplication, using nodal segments of 25- 30 years old trees i.e., promising interspecific F1 hybrid of Eucalyptus (Eucalyptus citriodora and Eucalyptus torelliana). Due to the phenolic exudation explants did not survive and eventually died without regenerating buds. The explants collected from July to September were the best for in vitro studies for icropropagation. The axillary buds were surface sterilized with 0.1% Mercuric chloride solution for 10-15 minutes, followed by 0.1% Bavistin treatment for 1 minute and subsequently washed 3-4 times with sterilized distilled water. These surface sterilized axillary buds were cultured on MS medium supplemented with cytokinin and auxin. MS medium supplemented with 1.5mg/l BAP + 0.1mg/l NAA proved to be the best hormonal combination for induction of axillary bud which resulted in the development of 1-3 axillary shoots. The proliferated shoots were cultured on MS medium with different concentration of BAP (0.1 – 3.0 mg/l) alone or in combination with NAA (0.1-1.5mg/l) and supplemented with sucrose at 3%. The aim of the paper was to obtain maximum rate of in vitro regeneration of FRI-15 by using micropropagation technique.