Cellular Functions Analyses Based on Nanorobotics

Abstract

Recently, Miyako group reported a new type of nano transporters that made of a liposome that decorated by a composition of carbon nanohorns (CNHs) and magnetic nanoparticles (MNPs) (Figure 1). This structure permeated into cells by a Neodymium magnet. Nano transporters not only could be moved by the magnetic field but also can be stimulated by NIR laser to control the release of liposome contains at a target place in organisms. This hybrid system has provided opportunities for analyses of biomolecular processes in organisms. As an achievement, enzymatic reaction controlled in the cancerous cells in vitro and transgene mice model in vivo [6]. β-galactosidase (β-Gal) is an enzyme that often over expressed in primary colorectal, breast, andovarian cancers [7]. The existence of this substance could be monitored by an enzymatic reaction in living cells. So, non-fluorescent fluorescein di-β-D-galactopyranoside (FDG) was loaded inside liposome part of the system.