l-Iditol production from l-sorbose by a methanol yeast, Candida boidinii (Kloeckera sp.) No. 2201

Journal of Fermentation Technology Pub Date : 1988-01-01 DOI:10.1016/0385-6380(88)90084-2

Vitchuporn Vongsuvanlert , Yoshiki Tani

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引用次数: 18

Abstract

Production of l-iditol (iditol) from l-sorbose (sorbose) with d-sorbitol dehydrogenase coupled with NADH regeneration under methanol oxidation was studied with the resting cell system of a methanol yeast, Candida boidinii (Kloeckera sp.) no. 2201.

Maximum activities of iditol production and enzymes concerned with the production were found in cells grown on a medium containing methanol and d-xylose (xylose). The highest amount of iditol, 142–148 g/l (94–98% conversion rate), was obtained from 150 g/l of sorbose in the presence of 0.5 M methanol at pH 6.5. Intermittent control of the pH during the cell reaction gave a significantly higher amount of iditol than that obtained without such control. Freeze-thawing cells showed rather higher productivity than resting cells. The product was identified as iditol without contamination of its C-2 epimer, d-sorbitol, by high performance liquid chromatography.

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用甲醇酵母，假丝酵母(Kloeckera sp.) 2201号从l-山梨糖生产l-二醇

利用甲醇酵母假丝酵母(Candida boidinii, Kloeckera sp.) no.)的静息细胞系统，研究了l-山梨糖脱氢酶与NADH再生在甲醇氧化条件下由l-山梨糖(sorbose)生产l-二醇(diitol)。2201.在含有甲醇和d-木糖(木糖)的培养基上生长的细胞中，发现了最大的二醇生产活性和与生产有关的酶。以150 g/l的山梨糖为原料，在0.5 M甲醇存在下，pH为6.5时，二醇的转化率最高，为142 ~ 148 g/l(转化率为94 ~ 98%)。在细胞反应过程中，间歇控制pH值所产生的二醇量明显高于没有这种控制的二醇量。冻融细胞的生产力明显高于静息细胞。通过高效液相色谱法鉴定产物为二醇，其C-2外聚物d-山梨醇未被污染。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of Fermentation Technology

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