Moxibustion on Carbonyl Stress and Expression of p19ARF and p53 mRNA in Livers of D-galactose-Induced Aging Rats

World Science and Technology Pub Date : 2010-12-01 DOI:10.1016/S1876-3553(11)60034-3

Ma Xiaopeng , Mo Wenquan , Zhao Chen , Wu Huangan , Zhou Cili , Zhang Shujing , Cui Yunhua , Tan Linying , Liu Huirong

{"title":"Moxibustion on Carbonyl Stress and Expression of p19ARF and p53 mRNA in Livers of D-galactose-Induced Aging Rats","authors":"Ma Xiaopeng , Mo Wenquan , Zhao Chen , Wu Huangan , Zhou Cili , Zhang Shujing , Cui Yunhua , Tan Linying , Liu Huirong","doi":"10.1016/S1876-3553(11)60034-3","DOIUrl":null,"url":null,"abstract":"<div>This study deals with the effect of moxibustion on carbonyl stress and the expression of p19ARF and p53 mRNA in livers of subacute aging rats. The SD rats were randomly divided into four groups: normal group, model group, moxibustion group, and Vit E group. Subacute aging rats were prepared with continuous high-dose intraperitoneal injection of D-galactose (D-gal). The moxibustion group received moxibustion at acupoint Shenshu (BL-23). Protein carbonyl content in livers was determined by biochemical chromatometry; p19ARF and p53 mRNA expressions were detected by fluorescence quantitative PCR. The results showed that compared with the normal group, protein carbonyl content, p19ARF, and p53 mRNA expressions were significantly higher in the model group (P < 0.01). Compared with the model group, protein carbonyl content, p19ARF, and p53 mRNA expressions were significantly lower in the moxibustion group and Vit E group (P < 0.01, P < 0.05). Our findings suggest that moxibustion at acupoint BL-23 reduced the protein carbonyl content, as well as the expression of p19ARF and p53 mRNA in livers of D-galactose-induced aging rats.</div>","PeriodicalId":101287,"journal":{"name":"World Science and Technology","volume":"12 6","pages":"Pages 892-896"},"PeriodicalIF":0.0000,"publicationDate":"2010-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1876-3553(11)60034-3","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"World Science and Technology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1876355311600343","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 1

Abstract

This study deals with the effect of moxibustion on carbonyl stress and the expression of p19ARF and p53 mRNA in livers of subacute aging rats. The SD rats were randomly divided into four groups: normal group, model group, moxibustion group, and Vit E group. Subacute aging rats were prepared with continuous high-dose intraperitoneal injection of D-galactose (D-gal). The moxibustion group received moxibustion at acupoint Shenshu (BL-23). Protein carbonyl content in livers was determined by biochemical chromatometry; p19ARF and p53 mRNA expressions were detected by fluorescence quantitative PCR. The results showed that compared with the normal group, protein carbonyl content, p19ARF, and p53 mRNA expressions were significantly higher in the model group (P < 0.01). Compared with the model group, protein carbonyl content, p19ARF, and p53 mRNA expressions were significantly lower in the moxibustion group and Vit E group (P < 0.01, P < 0.05). Our findings suggest that moxibustion at acupoint BL-23 reduced the protein carbonyl content, as well as the expression of p19ARF and p53 mRNA in livers of D-galactose-induced aging rats.

查看原文本刊更多论文

艾灸对d -半乳糖衰老大鼠肝脏羰基应激及p19ARF、p53 mRNA表达的影响

本研究探讨艾灸对亚急性衰老大鼠肝脏羰基应激及p19ARF、p53 mRNA表达的影响。将SD大鼠随机分为4组:正常组、模型组、艾灸组、维生素E组。采用连续大剂量腹腔注射d -半乳糖(D-gal)制备亚急性衰老大鼠。艾灸组在肾俞穴(BL-23)施灸。用生化色谱法测定肝脏蛋白质羰基含量;荧光定量PCR检测p19ARF和p53 mRNA的表达。结果显示，与正常组比较，模型组蛋白羰基含量、p19ARF、p53 mRNA表达均显著升高(P <0.01)。与模型组比较，艾灸组和Vit E组的蛋白羰基含量、p19ARF、p53 mRNA表达均显著降低(P <0.01, P <0.05)。我们的研究结果表明，灸BL-23穴可降低d -半乳糖诱导衰老大鼠肝脏中蛋白质羰基含量，以及p19ARF和p53 mRNA的表达。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

World Science and Technology

自引率

0.00%

发文量