Antioxidant enzymes immobilized on gold and silver nanoparticles enhance DNA repairing systems of rat skin after exposure to ultraviolet radiation

IF 4.2 2区医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL

Nanomedicine : nanotechnology, biology, and medicine Pub Date : 2022-07-01 DOI:10.1016/j.nano.2022.102558

Agnieszka M. Pudlarz Dr , Katarzyna Ranoszek-Soliwoda Dr hab. , Michał S. Karbownik Dr , Ewa Czechowska Dr , Emilia Tomaszewska Dr , Grzegorz Celichowski Prof. , Jarosław Grobelny Prof. , Ewa Chabielska Prof. , Anna Gromotowicz-Popławska Dr hab. , Janusz Szemraj Prof.

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引用次数: 3

Abstract

The aim of the study was to investigate in vivo whether the application of immobilized superoxide dismutase (SOD) and catalase (CAT) could enhance DNA repairing systems and reduce level of CPD (cyclobutane pyrimidine dimers) and 6-4PP ((6-4) pyrimidine-pyrimidone photoproducts), and whether the immobilization on gold (AuNPs) and silver (AgNPs) nanoparticles affects the outcome. The study presents secondary analysis of our previous research. Three-day application of SOD and CAT in all forms of solution decreased the levels of CPD and 6-4PP boosted by UV irradiation. The mRNA expression level of the nucleotide excision repair (NER) system genes (XPA, XPC, ERCC1, ERCC2, ERCC3, LIG1) increased after application of immobilized and free enzymes. Increased by UV irradiation, p53 mRNA expression level normalized with the enzyme application. In conclusion, application of free and immobilized antioxidant enzymes accelerates removal of harmful effects of UV radiation in the rat skin by increasing expression level of NER genes.

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金、银纳米颗粒固定化抗氧化酶增强紫外线照射后大鼠皮肤DNA修复系统

本研究的目的是研究体内应用固定化超氧化物歧化酶(SOD)和过氧化氢酶(CAT)是否能增强DNA修复系统，降低CPD(环丁烷嘧啶二聚体)和6-4PP((6-4)嘧啶-嘧啶光产物)的水平，以及金(AuNPs)和银(AgNPs)纳米颗粒固定化是否影响结果。本研究是对前人研究的二次分析。在所有形式的溶液中施用3 d的SOD和CAT降低了紫外线照射引起的CPD和6-4PP水平。应用固定化酶和游离酶后，核苷酸切除修复(NER)系统基因(XPA、XPC、ERCC1、ERCC2、ERCC3、LIG1) mRNA表达水平升高。在紫外线照射下，p53 mRNA的表达水平随着酶的使用而正常化。综上所述，游离和固定化抗氧化酶的应用通过提高NER基因的表达水平，加速了紫外线辐射对大鼠皮肤有害影响的清除。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nanomedicine : nanotechnology, biology, and medicine 工程技术-纳米科技

CiteScore

11.10

自引率

0.00%

发文量

133

审稿时长

42 days

期刊介绍： The mission of Nanomedicine: Nanotechnology, Biology, and Medicine (Nanomedicine: NBM) is to promote the emerging interdisciplinary field of nanomedicine. Nanomedicine: NBM is an international, peer-reviewed journal presenting novel, significant, and interdisciplinary theoretical and experimental results related to nanoscience and nanotechnology in the life and health sciences. Content includes basic, translational, and clinical research addressing diagnosis, treatment, monitoring, prediction, and prevention of diseases.