The effect of triiodothyronine on the hippocampal long-term potentiation in an animal model of the Alzheimer's disease: The role of BDNF and reelin

Q3 Medicine

Neurology Psychiatry and Brain Research Pub Date : 2019-09-01 DOI:10.1016/j.npbr.2019.07.004

Sahreh Shabani , Yaghoob Farbood , Alireza Sarkaki , Seyyed Ali Mard , Akram Ahangarpour , Layasadat Khorsandi

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引用次数: 6

Abstract

Converging evidence, propose a close relation between thyroid function and Alzheimer’s disease (AD). We have assessed the effect of subcutaneous and intrahippocampal administrations of triiodothyronine (T3) on the electrophysiological activity (hippocampal long-term potentiation (LTP)), the levels of thyroid hormones (THs) and TSH, the protein expression of BDNF and reelin as well as histological changes in the hippocampus of AD rats. Beta-amyloid (Aβ) plus ibotenic acid (Ibo) were injected intrahippocampally and rats were treated with T3 or saline. The hippocampal levels of THs and the protein expression are measured by ELISA kits and Western blotting method respectively. Results have been shown that T3 (S.C., and I. H), significantly reversed the amplitude and the slope impairment of the DG neurons, induced by Aβ. The hippocampal levels of THs, TSH and two protein expression were significantly decreased (p < 0.001) in AD animals and increased significantly in AD rats that have received T3 (S. C and I. H) (p < 0.01). Formation of amyloid plaques was declined in AD rats treated with T3. In conclusion, both S.C., and I.H. injections of T3 is effective in preventing the disruption of synaptic plasticity induced by Aβ. This positive effect of T3 may be mediated through a regulation of proteins expression and the hippocampal level of THs. The best effect was observed in I.H. microinjection of T3.

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三碘甲状腺原氨酸对阿尔茨海默病动物模型海马长期增强的影响:BDNF和reelin的作用

越来越多的证据表明，甲状腺功能与阿尔茨海默病(AD)密切相关。我们评估了皮下和海马内给药三碘甲状腺原氨酸(T3)对AD大鼠海马电生理活动(海马长期增强(LTP))、甲状腺激素(THs)和TSH水平、BDNF和reelin蛋白表达以及组织学变化的影响。大鼠海马内注射β -淀粉样蛋白(Aβ)和伊博tenic酸(Ibo)，并给予T3或生理盐水处理。分别用ELISA试剂盒和Western blotting法检测海马组织中三萜类化合物的含量和蛋白表达。结果表明，T3 (S.C和i.h)能显著逆转Aβ诱导的DG神经元振幅和斜率损伤。AD动物海马中THs、TSH水平及两种蛋白表达均显著降低(p < 0.001)，而T3 (S. C和I. H) AD大鼠海马中THs、TSH水平及两种蛋白表达均显著升高(p < 0.01)。经T3处理的AD大鼠淀粉样斑块的形成减少。综上所述，S.C和I.H.注射T3均能有效预防Aβ引起的突触可塑性破坏。T3的这种积极作用可能是通过调节蛋白质表达和海马THs水平来介导的。体外微注射T3效果最好。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Neurology Psychiatry and Brain Research 医学-精神病学

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期刊介绍： Neurology, Psychiatry & Brain Research publishes original papers and reviews in biological psychiatry, brain research, neurology, neuropsychiatry, neuropsychoimmunology, psychopathology, psychotherapy. The journal has a focus on international and interdisciplinary basic research with clinical relevance. Translational research is particularly appreciated. Authors are allowed to submit their manuscript in their native language as supplemental data to the English version. Neurology, Psychiatry & Brain Research is related to the oldest German speaking journal in this field, the Centralblatt fur Nervenheilkunde, Psychiatrie und gerichtliche Psychopathologie, founded in 1878. The tradition and idea of previous famous editors (Alois Alzheimer and Kurt Schneider among others) was continued in modernized form with Neurology, Psychiatry & Brain Research. Centralblatt was a journal of broad scope and relevance, now Neurology, Psychiatry & Brain Research represents a journal with translational and interdisciplinary perspective, focusing on clinically oriented research in psychiatry, neurology and neighboring fields of neurosciences and psychology/psychotherapy with a preference for biologically oriented research including basic research. Preference is given for papers from newly emerging fields, like clinical psychoimmunology/neuroimmunology, and ideas.