Comparison various level ascorbic acid and lycopene additions in semen diluent enhanced sperm quality of Sapudi ram.

IF 2.7 3区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of Animal Science and Technology Pub Date : 2024-09-01 Epub Date: 2024-09-30 DOI:10.5187/jast.2023.e54

Bintara Sigit, Dyah Maharani, Luis Tavares, Pradita Iustitia Sitaresmi

{"title":"Comparison various level ascorbic acid and lycopene additions in semen diluent enhanced sperm quality of Sapudi ram.","authors":"Bintara Sigit, Dyah Maharani, Luis Tavares, Pradita Iustitia Sitaresmi","doi":"10.5187/jast.2023.e54","DOIUrl":null,"url":null,"abstract":"The primary cause of sperm quality decline during the freeze-thaw pathway is the peroxidation hazard caused by reactive oxygen species produced by the biological molecules of sperm. Ascorbic acid (Vitamin C) and lycopene are two potent antioxidants that operate to prevent oxidation processes. This study aimed to analyse the effects of ascorbic acid and lycopene on the motility, viability, abnormality and plasma membrane integrity of post-thawed Sapudi rams. Sperm samples were obtained and pooled from six sexually mature Sapudi rams, separated into ten equal proportions and diluted with Tris-egg yolk-glycerol (TEY) extender. Semen was supplemented with 0 (C0; L0), 1 (C1; L1), 2 (C2; L2), 3 (C3; L3) and 4 (C4; L4) mg/100 mL (1%-4%) diluent each of ascorbic acid and lycopene, respectively. Total sperm motility, viability, abnormalities and semen membrane plasma (%) were analysed after thawing. C3 and L3 extenders resulted in higher total motility (p < 0.05) compared to the other extenders, with all treatments higher than that of the control. The extender C3 (p < 0.05) exhibited the highest semen quality. Finally, the current findings show that C3 and L3 can increase the quality of post-thawed Sapudi ram spermatozoa.","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":null,"pages":null},"PeriodicalIF":2.7000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11466734/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Animal Science and Technology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.5187/jast.2023.e54","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/9/30 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}

引用次数: 0

Abstract

The primary cause of sperm quality decline during the freeze-thaw pathway is the peroxidation hazard caused by reactive oxygen species produced by the biological molecules of sperm. Ascorbic acid (Vitamin C) and lycopene are two potent antioxidants that operate to prevent oxidation processes. This study aimed to analyse the effects of ascorbic acid and lycopene on the motility, viability, abnormality and plasma membrane integrity of post-thawed Sapudi rams. Sperm samples were obtained and pooled from six sexually mature Sapudi rams, separated into ten equal proportions and diluted with Tris-egg yolk-glycerol (TEY) extender. Semen was supplemented with 0 (C0; L0), 1 (C1; L1), 2 (C2; L2), 3 (C3; L3) and 4 (C4; L4) mg/100 mL (1%-4%) diluent each of ascorbic acid and lycopene, respectively. Total sperm motility, viability, abnormalities and semen membrane plasma (%) were analysed after thawing. C3 and L3 extenders resulted in higher total motility (p < 0.05) compared to the other extenders, with all treatments higher than that of the control. The extender C3 (p < 0.05) exhibited the highest semen quality. Finally, the current findings show that C3 and L3 can increase the quality of post-thawed Sapudi ram spermatozoa.

查看原文本刊更多论文

在精液稀释液中添加不同水平的抗坏血酸和番茄红素可提高萨普迪公羊的精子质量。

冷冻-解冻过程中精子质量下降的主要原因是精子生物分子产生的活性氧造成的过氧化危害。抗坏血酸（维生素 C）和番茄红素是两种有效的抗氧化剂，可防止氧化过程。本研究旨在分析抗坏血酸和番茄红素对解冻后萨普迪公羊精子的活力、存活率、畸形率和质膜完整性的影响。从六只性成熟的萨普迪公羊身上获取精子样本并将其汇集，分成十等份并用三蛋黄-甘油（TEY）扩展剂稀释。精液中分别添加了 0（C0；L0）、1（C1；L1）、2（C2；L2）、3（C3；L3）和 4（C4；L4）毫克/100 毫升（1%-4%）的抗坏血酸和番茄红素稀释剂。解冻后分析了精子的总活力、存活率、畸形率和精液膜浆（%）。与其他扩展剂相比，C3 和 L3 扩展剂使精子总活力更高（p < 0.05），所有处理的精子总活力均高于对照组。C3延长剂的精液质量最高（p < 0.05）。最后，目前的研究结果表明，C3 和 L3 可以提高解冻后萨普迪公羊精子的质量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Animal Science and Technology Agricultural and Biological Sciences-Food Science

CiteScore

4.50

自引率

8.70%

发文量

审稿时长

7 weeks

期刊介绍： Journal of Animal Science and Technology (J. Anim. Sci. Technol. or JAST) is a peer-reviewed, open access journal publishing original research, review articles and notes in all fields of animal science. Topics covered by the journal include: genetics and breeding, physiology, nutrition of monogastric animals, nutrition of ruminants, animal products (milk, meat, eggs and their by-products) and their processing, grasslands and roughages, livestock environment, animal biotechnology, animal behavior and welfare. Articles generally report research involving beef cattle, dairy cattle, pigs, companion animals, goats, horses, and sheep. However, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will also be considered for publication. The Journal of Animal Science and Technology (J. Anim. Technol. or JAST) has been the official journal of The Korean Society of Animal Science and Technology (KSAST) since 2000, formerly known as The Korean Journal of Animal Sciences (launched in 1956).