Molecular analysis of genetic diversity of Machilus odoratissima nees species collected from central and highland areas of Vietnam

Abstract

In this study, we analyzed the genetic diversity of 50 Machilus odoratissima Nees sample species collected from the central and highland areas of Vietnam by using 13 SSR markers. The results showed that that 54 different alleles (approximately 4.15 alleles/locus), in which 42 alleles were polymorphism (80.77%), 10 alleles were monomorphism (19.23%). The PIC value was varied from 0.63 to 0.84 (average value: 0.70), respectively. The rate of homogenates among the samples were ranged from 0.63 to 0.98 (average value 0.81). The 50 samples were divided into 5 different groups based on the genetic relationships: group I (10 samples) included the samples were collected in Quang Tri, Quang Nam and Gia Lai provinces; group II (5 samples) collected in Quang Nam and Quang Tri provinces; group III (05 samples) were collected in Thua Thien Hue and Kon Tum provinces; and the group IV (20 samples) were collected in Quang Tri, Gia Lai and Kon Tum provinces (the largest group (40%); the group V (10 samples) collected in Thua Thien Hue, Kon Tum and Gia Lai provinces. The results demonstrated that the rate of homogeneity was quite high in M. odoratissima Nees species collected in the areas of central and highland of Vietnam. This study may provide good information for further identification of high-quality genetic germ48 Dang Thi Thanh Ha et al. plasm of the medical plant resource for development and conservation in this country.