Extraction and comparison of quality and purity of DNA of medicinal plants by CTAB and kit method

Raheela Jabeen, Ume Habiba, Sana Khalid, Jafir Hussain Shirazi
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Abstract

Background: Today is a world of bioengineering. Man is playing with DNA. Extraction and isolation of DNA has become as a key part for the researches of modern times. One needs high quality and increased quantity of DNA for performing several genetic techniques. There are several methods through which DNA can be extracted but the quality of the DNA cannot be compromised. Material methods: In the current research work extraction of DNA from four medicinal important plants Cassia fistula, Saccharum officinarum, Albizzia lebbeck and Cymbopogan citratus was carried out by two methods. One is a manual method that is used for the extraction of DNA from plant source; The CTAB method, and the other is extraction with the help of commercially available kit. Results: Results of the work showed that the high quantity of the DNA was obtained by CTAB method while DNA extracted with the kit was of better quality and purity then CTAB method. Conclusion: In techniques where high quality DNA is required like genetic engineering, micro arrays, extraction of DNA through kit method should be preferred, while in cases where quantity of DNA maters over its quality CTAB method is better to be used and economic.
CTAB法和试剂盒法提取药用植物DNA的质量和纯度比较
背景:今天是一个生物工程的世界。人类在玩弄DNA。DNA的提取与分离已成为现代医学研究的重要组成部分。人们需要高质量和更多数量的DNA来执行一些遗传技术。有几种方法可以提取DNA,但不能损害DNA的质量。材料方法:在目前的研究工作中,采用两种方法提取了决明子、白糖、合欢和香茅四种重要药用植物的DNA。一种是从植物源中提取DNA的人工方法;一种是CTAB法,另一种是在市售试剂盒的帮助下提取。结果:实验结果表明,CTAB法获得的DNA量高,试剂盒提取的DNA质量和纯度优于CTAB法。结论:在基因工程、微阵列等对DNA质量要求较高的技术中,应优先采用试剂盒法提取DNA,而在DNA数量比质量更重要的情况下,采用CTAB法提取DNA更为经济。
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