S2.4d Combination antifungal effects of eugenol with voriconazole against Candida tropicalis and Candida krusei strains isolated from the genital tract of mares

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-09-01 DOI:10.1093/mmy/myac072.S2.4d

A. Sharifzadeh, H. Shokri, D. Nikaein

{"title":"S2.4d Combination antifungal effects of eugenol with voriconazole against Candida tropicalis and Candida krusei strains isolated from the genital tract of mares","authors":"A. Sharifzadeh, H. Shokri, D. Nikaein","doi":"10.1093/mmy/myac072.S2.4d","DOIUrl":null,"url":null,"abstract":"Abstract S2.4 Veterinary mycology research, September 21, 2022, 3:00 PM - 4:30 PM Objectives The objective of the current study was to investigate the possibility that eugenol synergizes the antifungal effects of voriconazole on genital isolates of Candida krusei and Candida tropicalis from mares. Methods The antifungal activity of eugenol and voriconazole was evaluated using the broth microdilution assay (CLSI- M27-A3). The synergism of eugenol and voriconazole against genital Candida isolates was evaluated by the microdilution checkerboard method. Results Minimum inhibitory concentration (MIC) values for eugenol and voriconazole ranged from 400 to 800 μg/ml and 1 to 8 μg/ml, respectively, for C. tropicalis isolates, and from 200 to 400 μg/ml for eugenol and 2 to 16 μg/ml for voriconazole against C. krusei isolates. Eugenol decreased the arithmetic mean of MIC for voriconazole against C. tropicalis and C. krusei isolates from 2.66 to 0.46 μg/ml and 7.77 to 0.41 μg/ml respectively. The fractional inhibitory concentration index (FICI) values for the eugenol voriconazole combination ranged from 0.25 to 0.88 and 0.19 to 0.63 for C. tropicalis and C. krusei isolates respectively. A synergistic effect of eugenol in combination with voriconazole was observed for 83.3% of C. tropicalis and 77.7% of C. krusei isolates. The antagonistic activity was not seen in any of the isolates tested. Conclusions Eugenol showed fungistatic and fungicidal effects against genital Candida isolates and, in combination, synergized the antifungal effects of voriconazole. The eugenol-voriconazole combination can lay the foundation for a therapeutic approach against isolates in which azole resistance has increased over time.","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1093/mmy/myac072.S2.4d","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

Abstract S2.4 Veterinary mycology research, September 21, 2022, 3:00 PM - 4:30 PM Objectives The objective of the current study was to investigate the possibility that eugenol synergizes the antifungal effects of voriconazole on genital isolates of Candida krusei and Candida tropicalis from mares. Methods The antifungal activity of eugenol and voriconazole was evaluated using the broth microdilution assay (CLSI- M27-A3). The synergism of eugenol and voriconazole against genital Candida isolates was evaluated by the microdilution checkerboard method. Results Minimum inhibitory concentration (MIC) values for eugenol and voriconazole ranged from 400 to 800 μg/ml and 1 to 8 μg/ml, respectively, for C. tropicalis isolates, and from 200 to 400 μg/ml for eugenol and 2 to 16 μg/ml for voriconazole against C. krusei isolates. Eugenol decreased the arithmetic mean of MIC for voriconazole against C. tropicalis and C. krusei isolates from 2.66 to 0.46 μg/ml and 7.77 to 0.41 μg/ml respectively. The fractional inhibitory concentration index (FICI) values for the eugenol voriconazole combination ranged from 0.25 to 0.88 and 0.19 to 0.63 for C. tropicalis and C. krusei isolates respectively. A synergistic effect of eugenol in combination with voriconazole was observed for 83.3% of C. tropicalis and 77.7% of C. krusei isolates. The antagonistic activity was not seen in any of the isolates tested. Conclusions Eugenol showed fungistatic and fungicidal effects against genital Candida isolates and, in combination, synergized the antifungal effects of voriconazole. The eugenol-voriconazole combination can lay the foundation for a therapeutic approach against isolates in which azole resistance has increased over time.

查看原文本刊更多论文

S2.4d丁香酚联合伏立康唑对母马生殖道热带念珠菌和克鲁氏念珠菌的联合抑菌作用

摘要/ Abstract摘要:兽医真菌学研究，2022年9月21日，下午3:00 - 4:30目的探讨丁香酚协同伏立康唑对母马生殖道分离株克鲁假丝酵母菌和热带假丝酵母菌的抑菌作用。方法采用肉汤微量稀释法(CLSI- M27-A3)测定丁香酚和伏立康唑的抑菌活性。采用微量稀释棋盘法评价丁香酚和伏立康唑对生殖道念珠菌的增效作用。结果丁香酚和伏立康唑对热带镰刀菌的最小抑制浓度(MIC)分别为400 ~ 800 μg/ml和1 ~ 8 μg/ml，丁香酚和伏立康唑对克鲁塞镰刀菌的最小抑制浓度(MIC)分别为200 ~ 400 μg/ml和2 ~ 16 μg/ml。丁香酚可使伏立康唑对热带恙虫病和克氏恙虫病分离株的MIC算术平均值分别从2.66降至0.46 μg/ml和7.77降至0.41 μg/ml。丁香酚伏立康唑联合抑菌浓度指数(FICI)分别为0.25 ~ 0.88和0.19 ~ 0.63。丁香酚与伏立康唑联用对83.3%的热带恙虫和77.7%的克氏恙虫有增效作用。拮抗活性未在所有分离株中发现。结论丁香酚对生殖道念珠菌有抑菌和杀菌作用，与伏立康唑联用可协同抑菌作用。丁香酚-伏立康唑联合用药可以为针对对唑的耐药性随时间增加的分离株的治疗方法奠定基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.