Ultrasound-guided chemoradiotherapy of breast cancer using smart methotrexate-loaded perfluorohexane nanodroplets.

IF 4.2 2区医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL

Nanomedicine : nanotechnology, biology, and medicine Pub Date : 2022-12-01 DOI:10.2139/ssrn.4210211

R. K. Samani, Fatemeh Maghsoudinia, Fatemeh Mehradnia, S. Hejazi, M. Saeb, Tayebe Sobhani, Zohreh Farahbakhsh, M. Mehrgardi, M. Tavakoli

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引用次数: 2

Abstract

Chemoradiotherapy with controlled-release nanocarriers such as sono-sensitive nanodroplets (NDs) can enhance the anticancer activity of chemotherapy medicines and reduces normal tissue side effects. In this study, folic acid-functionalized methotrexate-loaded perfluorohexane NDs with alginate shell (FA-MTX/PFH@alginate NDs) were synthesized, characterized, and their potential for ultrasound-guided chemoradiotherapy of breast cancer was investigated in vitro and in vivo. The cancer cell (4T1) viabilities and surviving fractions after NDs and ultrasound treatments were significantly decreased. However, this reduction was much more significant for ultrasound in combination with X-ray irradiation. The in vitro and in vivo results confirmed that MTX-loaded NDs are highly biocompatible and they have no significant hemolytic activity and organ toxicity. Furthermore, the in vivo results indicated that the FA-MTX/PFH@alginate NDs were accumulated selectively in the tumor region. In conclusion, FA-functionalized MTX/PFH@alginate NDs have a great theranostic performance for ultrasound-controlled drug delivery in combination with radiotherapy of breast cancer.

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使用装载智能甲氨蝶呤的全氟己烷纳米液滴的超声引导乳腺癌放化疗。

利用声敏纳米液滴(NDs)等可控释放纳米载体进行放化疗，可以增强化疗药物的抗癌活性，减少正常组织的副作用。本研究合成了叶酸功能化的海藻酸壳甲氨蝶呤负载全氟己烷NDs (FA-MTX/PFH@alginate NDs)，并对其进行了表征，并在体外和体内研究了其在超声引导乳腺癌放化疗中的潜力。NDs和超声治疗后肿瘤细胞(4T1)存活率和存活分数显著降低。然而，当超声与x射线联合照射时，这种减少更为显著。体外和体内实验结果证实，含mtx的NDs具有高度的生物相容性，没有明显的溶血活性和器官毒性。此外，体内结果表明FA-MTX/PFH@alginate NDs在肿瘤区域选择性积累。综上所述，fa功能化的MTX/PFH@alginate NDs在乳腺癌的超声控制给药联合放疗中具有良好的治疗效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nanomedicine : nanotechnology, biology, and medicine 工程技术-纳米科技

CiteScore

11.10

自引率

0.00%

发文量

133

审稿时长

42 days

期刊介绍： The mission of Nanomedicine: Nanotechnology, Biology, and Medicine (Nanomedicine: NBM) is to promote the emerging interdisciplinary field of nanomedicine. Nanomedicine: NBM is an international, peer-reviewed journal presenting novel, significant, and interdisciplinary theoretical and experimental results related to nanoscience and nanotechnology in the life and health sciences. Content includes basic, translational, and clinical research addressing diagnosis, treatment, monitoring, prediction, and prevention of diseases.