Evaluation of the effectiveness of PCR test systems for monitoring decontamination of environmental objects potentially infected with SARS-CoV-2 coronavirus

Abstract

In the context of the coronavirus pandemic, one of the priority tasks is the disinfection of environmental objects in order to comply with the anti-epidemic regime. In this regard, questions arise regarding the laboratory control of the effectiveness of disinfection measures. The use of virological approaches is hardly acceptable for solving this problem, and the use of fast and affordable methods of molecular genetic analysis comes to the fore, which are certainly of signal value, but sufficient to evaluate the effectiveness of these measures. In this work, studies were carried out on the detection of SARS-CoV-2 coronavirus RNA by several test systems using the real-time polymerase chain reaction method in swabs from various surfaces after they were treated with a disinfectant. The study showed that to control the decontamination of environmental objects potentially infected with the SARS-CoV-2 coronavirus, test systems can be used to detect the RNA of the SARS-CoV-2 virus in clinical samples. The greatest sensitivity is shown for PCR kits in which multichannel detection of individual RNA sites is carried out. Keywords: disinfectants, decontamination control, novel coronavirus infection COVID-19.