Comparative Forensic Characterization of Blood Components by Increased Alcoholic Concentrations

Abstract

BAC accurate and precise levels are important for forensic studies [1]. The sample collection and various methods adopted for determining blood alcohol concentrations is an important tool in forensic science and toxicological studies [2]. The differences in the concentrations of plasma and whole blood are discussed in this report. It has been reported earlier that whole blood has less water content as compared to plasma [3]. The reported ratio of the two is 1.0 1.1 [4]. The objective is to maintain the blood samples and analyze them against alcohol concentrations. In the vials used for collecting samples, preservatives and anticoagulants are added and then stored at different temperatures. In the in vivo techniques [5]. various difficulties are there. EDTA and Sodium fluoride are common preservatives for blood samples [6,7]. This report summarizes the role and effects of coagulant on BAC at concentrations of 30, 50, and 100 mg/dL from 2 mL ethanol, and n-propyl alcohol was taken as the internal standard. An aqueous solution of sodium tungstate (10% w/v) was used to precipitate the proteins. Plasma samples were centrifuged for 8 min at 3200 rpm at room temperature. Samples were extracted using 300 μL each of internal standard, adding 10% sodium tungstate. Extractions were centrifuged at 3500 rpm for 5 min at 35°C and vials were set for instrumentation. Material and methods