The Use of Photographic Color Information for High-Throughput Phenotyping of Pigment Composition in Agarophyton vermiculophyllum (Ohmi) Gurgel, J.N.Norris & Fredericq

IF 1.4 4区生物学 Q3 MARINE & FRESHWATER BIOLOGY

Cryptogamie Algologie Pub Date : 2019-09-16 DOI:10.5252/cryptogamie-algologie2019v40a7

W. Ryan, S. Heiser, M. Curtis, C. Amsler, T. Bayer, G. Bonthond, Gaoge Wang, F. Weinberger, S. A. Krueger‐Hadfield

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引用次数: 0

Abstract

ABSTRACT Pigment variation within and among algal species may have important ecological consequences because small changes in the concentration and composition of pigments can influence the photosynthetic efficiency and rate as well as the spectra of light utilized. Toward the goal of developing a rapid method for comparing pigment composition among algal thalli, we characterized the relationship between visual color information taken from photographs (e.g., red, green, and blue color values) and photopigment composition in the non-native red alga Agarophyton vermiculophyllum (Ohmi) Gurgel, J.N.Norris & Fredericq. We used a set of 19 thalli, collected from across the known native and non-native range in the Northern Hemisphere, which exhibited substantial color variation at the time of field collection, and sustained this variation after being maintained in a common garden. We identified a set of ecologically interesting pigment traits that are readily predicted by color information, including chlorophyll a and phycobilin concentration. Finally, we demonstrated the repeatability of estimating color phenotypes from photographs of thalli taken under a range of light conditions in order to evaluate the utility of this approach for field studies. We suggest this method could be useful for the rapid, high-throughput phenotyping of photopigments in other red algae as well.

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利用照相颜色信息对线虫色素组成进行高通量表型分析

藻类物种内部和物种之间的色素变化可能会产生重要的生态后果，因为色素浓度和组成的微小变化会影响光合效率和速率以及利用的光谱。为了开发一种快速比较藻体色素组成的方法，我们描述了从照片中获取的视觉颜色信息(例如，红色，绿色和蓝色值)与非本地红藻Agarophyton vermiculophyllum (Ohmi)的光色素组成之间的关系。我们使用了一组19个从北半球已知的本地和非本地范围收集的菌体，这些菌体在野外采集时表现出明显的颜色变化，并且在一个共同的花园中养护后保持了这种变化。我们发现了一组生态上有趣的色素性状，这些性状很容易通过颜色信息来预测，包括叶绿素a和藻胆素浓度。最后，我们展示了在一系列光照条件下从菌体照片中估计颜色表型的可重复性，以评估该方法在实地研究中的实用性。我们认为该方法也可用于其他红藻光色素的快速、高通量表型分析。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cryptogamie Algologie 生物-海洋与淡水生物学

CiteScore

2.60

自引率

7.70%

发文量

审稿时长

>12 weeks

期刊介绍： Cryptogamie is a fast-track and peer-reviewed journal of international scope publishing in English only. It accepts original papers and review articles on the taxonomy, biology and ecology of all cryptogams. An issue of Cryptogamie may be devoted to a single topic, under the responsibility of guest editor(s). All articles published in Cryptogamie are compliant with the different nomenclatural codes. A copyright assignment will be signed by the authors before publication. Cryptogamie, Algologie accepts articles on systematics as well as ecology and evolution of any kind of algae (including Cyanobacteria).