Study on imperatorin extracted from Angelica dahurica and its UV photocatalytic reaction with collagen

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-01-20 DOI:10.3233/mgc-210179

W. Jiang, Xiaohua Zhou, Ke Ni

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Abstract

Soxhlet extraction method was used to extract imperatorin from Angelica dahurica, and the extraction ratio under different extraction condition was optimized to attain the best condition. Then, XAD-16 macroporous resin was selected as the optimal resin to boost the extraction ratio of imperatorin. Afterwards, the higher purity of imperatorin (96.84±0.2%) was separated by preparative HPLC system. Next, the photocatalytic reaction between the above imperatorin and collagen which the highest levels in skin was investigated using UV-vis spectroscopy, amino acid analysis and HPLC analysis. The results showed that imperatorin reacted with collagen only under ultraviolet light which caused the denaturation of collagen, and three new products were generated. The ultraviolet products were isolated by preparative HPLC system and separately detected by high-resolution mass spectrum. The possible UV photocatalytic reaction mechanism between imperatorin and collagen is that ultraviolet light induces the increase of the activity of the imperatorin to react with the tyrosine in the collagen, resulted in the denaturation of collagen and reestablish of the normal epidermal tissue in skin.

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白芷中欧前胡素的提取及其与胶原蛋白的紫外光催化反应研究

采用索氏提取法提取白芷中的欧胡素，并对不同提取条件下的提取率进行优化，得到最佳提取条件。以XAD-16大孔树脂为最佳树脂，提高了欧前胡素的提取率。制备型高效液相色谱分离得到较高纯度的欧前胡素(96.84±0.2%)。其次，利用紫外-可见光谱、氨基酸分析和高效液相色谱分析研究了上述欧前胡素与皮肤中含量最高的胶原蛋白之间的光催化反应。结果表明，欧前胡素仅在紫外光下与胶原发生反应，使胶原变性，生成3种新产物。紫外产物采用制备型高效液相色谱分离，高分辨率质谱分离检测。欧前胡素与胶原蛋白的紫外光催化反应机制可能是紫外光诱导欧前胡素活性的增加，与胶原蛋白中的酪氨酸发生反应，导致胶原变性，重建皮肤正常表皮组织。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.