Effect of pretreatment with organic solvent on enzymatic digestibility of cauliflower wastes

S. Majumdar, B. Goswami, Ankita Chakraborty, D. Bhattacharyya, J. Bhowal
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引用次数: 6

Abstract

Abstract The present study investigated the operational conditions for different pretreatment approaches and subsequent enzymatic hydrolysis of cauliflower wastes (stalk and leaf) for better release of fermentable sugars. The structural analysis of raw and pretreated lignocellulosic biomasses was investigated using scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transforms infrared (FTIR) analysis. Results demonstrated that the highest cellulose conversion rate and removal of most of the hemicellulose and lignin were obtained with organosolvent pretreatment. Using methanol in presence of sodium (Na) acetate was most effective in delignification of cauliflower wastes. In the present study, methanol (100% v/v) in presence of 0.1 M Na-acetate at 121 °C for 45 and 60 min for stalk and leaf, respectively, gave maximum reducing sugar yield. Response surface methodology was used to optimize different process parameters for enzymatic saccharification using microbial cellulase and xylanase. The optimum operation condition of enzymatic hydrolysis of organosolvent pretreated cauliflower wastes were substrate loading (2.5% w/v for both stalk and leaf), enzyme loading (15 and 10 U/g for stalk and leaf, respectively), pH (4.46 and 5.48 for stalk and leaf, respectively), at 60 °C and for 180 min.
有机溶剂预处理对菜花废酶消化率的影响
摘要本研究研究了不同预处理方法的操作条件,并对花椰菜废弃物(茎叶)进行酶解,以更好地释放可发酵糖。采用扫描电镜(SEM)、x射线衍射(XRD)和傅里叶变换红外(FTIR)分析对原料和预处理木质纤维素生物质进行了结构分析。结果表明,有机溶剂预处理能获得最高的纤维素转化率和大部分半纤维素和木质素的去除率。甲醇在乙酸钠存在下对花椰菜废渣进行脱木质素处理是最有效的。在本研究中,甲醇(100% v/v)在0.1 M Na-acetate的存在下,在121°C下,对茎和叶分别处理45和60 min,可获得最大的还原糖产量。采用响应面法对微生物纤维素酶和木聚糖酶的糖化工艺参数进行优化。有机溶剂预处理菜花废弃物酶解的最佳操作条件为底物量(茎叶均为2.5% w/v)、酶量(茎叶分别为15和10 U/g)、pH(茎叶分别为4.46和5.48)、60℃、180 min。
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