The Effect of Continued Training with Crocin on Apoptosis Markers in Liver Tissue of High Fat Diet Induced Diabetic Rats

Q4 Biochemistry, Genetics and Molecular Biology

Molecular & Cellular Biomechanics Pub Date : 2020-01-01 DOI:10.32604/mcb.2020.011532

Zahra Alimanesh, Zeynab Alimanesh, F. Davari, S. - Shadmehri, M. Ahmadi, S. Hosseini, S. Dolati, Alemeh Hariri Far

{"title":"The Effect of Continued Training with Crocin on Apoptosis Markers in Liver Tissue of High Fat Diet Induced Diabetic Rats","authors":"Zahra Alimanesh, Zeynab Alimanesh, F. Davari, S. - Shadmehri, M. Ahmadi, S. Hosseini, S. Dolati, Alemeh Hariri Far","doi":"10.32604/mcb.2020.011532","DOIUrl":null,"url":null,"abstract":": Diabetes mellitus (DM) disease can affect process of apoptosis by increasing oxidative stress, nevertheless exercise and crocin can improve apoptosis; therefore present study aimed to investigate the effect of continued training with crocin on apoptosis markers in liver tissue of diabetic rats. In this experimental study 32 diabetic rats based on fasting glucose divided into four groups of eight rats including: 1) sham, 2) training, 3) crocin, and 4) training with crocin also for investigate the effect of DM induction on apoptosis markers, eight healthy rats assigned in healthy control group. During eight weeks groups 2 and 4 ran 60 minutes on treadmill with intensity of 50 – 55% maximum speed for three sessions per week and groups 3 and 4 received 25 mg/kg/day crocin peritoneally. Shapiro ’ post-hot statistical analysis of data (P ≤ 0.05) . DM induction signi ﬁ cantly increased Bcl-2 as well as decreased Bax and P52 (P ≤ 0.05) nevertheless training and training with crocin signi ﬁ cantly decreased Bcl-2 and increased Bax and P53 (P ≤ 0.05) ; crocin signi ﬁ cantly decreased Bcl-2 and increased P53 (P ≤ 0.05) and training with crocin had higher effect on increase of Bax and P53 compare to training (P ≤ 0.05) also increase of Bax compare to crocin. Although training and crocin alone can improve apoptotic markers in diabetic rats, nevertheless training simultaneously with crocin have better effects than training alone.","PeriodicalId":48719,"journal":{"name":"Molecular & Cellular Biomechanics","volume":"44 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular & Cellular Biomechanics","FirstCategoryId":"1087","ListUrlMain":"https://doi.org/10.32604/mcb.2020.011532","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}

引用次数: 4

Abstract

: Diabetes mellitus (DM) disease can affect process of apoptosis by increasing oxidative stress, nevertheless exercise and crocin can improve apoptosis; therefore present study aimed to investigate the effect of continued training with crocin on apoptosis markers in liver tissue of diabetic rats. In this experimental study 32 diabetic rats based on fasting glucose divided into four groups of eight rats including: 1) sham, 2) training, 3) crocin, and 4) training with crocin also for investigate the effect of DM induction on apoptosis markers, eight healthy rats assigned in healthy control group. During eight weeks groups 2 and 4 ran 60 minutes on treadmill with intensity of 50 – 55% maximum speed for three sessions per week and groups 3 and 4 received 25 mg/kg/day crocin peritoneally. Shapiro ’ post-hot statistical analysis of data (P ≤ 0.05) . DM induction signi ﬁ cantly increased Bcl-2 as well as decreased Bax and P52 (P ≤ 0.05) nevertheless training and training with crocin signi ﬁ cantly decreased Bcl-2 and increased Bax and P53 (P ≤ 0.05) ; crocin signi ﬁ cantly decreased Bcl-2 and increased P53 (P ≤ 0.05) and training with crocin had higher effect on increase of Bax and P53 compare to training (P ≤ 0.05) also increase of Bax compare to crocin. Although training and crocin alone can improve apoptotic markers in diabetic rats, nevertheless training simultaneously with crocin have better effects than training alone.

查看原文本刊更多论文

持续用藏红花素训练对高脂饮食诱导的糖尿病大鼠肝组织凋亡标志物的影响

糖尿病(DM)可通过增加氧化应激影响细胞凋亡过程，而运动和藏红花素可促进细胞凋亡;因此，本研究旨在探讨藏红花素持续训练对糖尿病大鼠肝组织凋亡标志物的影响。本实验以32只空腹血糖为基础，将糖尿病大鼠分为4组，每组8只，分别为:1)假手术组，2)训练组，3)藏红花素组，4)藏红花素训练组，研究DM诱导对细胞凋亡标志物的影响，8只健康大鼠作为健康对照组。在8周内，第2组和第4组在跑步机上以最大速度50 - 55%的强度跑步60分钟，每周3次，第3组和第4组腹腔注射25 mg/kg/天的藏红花素。夏皮罗热后数据统计分析(P≤0.05)。DM诱导显著升高Bcl-2，降低Bax和P52 (P≤0.05)，而训练和藏红花素联合训练显著降低Bcl-2，升高Bax和P53 (P≤0.05);藏红花素能显著降低Bcl-2，升高P53 (P≤0.05)，且训练对Bax和P53的升高作用高于训练(P≤0.05)，Bax的升高作用高于藏红花素(P≤0.05)。虽然单独训练和藏红花素可以改善糖尿病大鼠的凋亡标志物，但同时训练和藏红花素比单独训练效果更好。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Molecular & Cellular Biomechanics CELL BIOLOGYENGINEERING, BIOMEDICAL&-ENGINEERING, BIOMEDICAL

CiteScore

1.70

自引率

0.00%

发文量

期刊介绍： The field of biomechanics concerns with motion, deformation, and forces in biological systems. With the explosive progress in molecular biology, genomic engineering, bioimaging, and nanotechnology, there will be an ever-increasing generation of knowledge and information concerning the mechanobiology of genes, proteins, cells, tissues, and organs. Such information will bring new diagnostic tools, new therapeutic approaches, and new knowledge on ourselves and our interactions with our environment. It becomes apparent that biomechanics focusing on molecules, cells as well as tissues and organs is an important aspect of modern biomedical sciences. The aims of this journal are to facilitate the studies of the mechanics of biomolecules (including proteins, genes, cytoskeletons, etc.), cells (and their interactions with extracellular matrix), tissues and organs, the development of relevant advanced mathematical methods, and the discovery of biological secrets. As science concerns only with relative truth, we seek ideas that are state-of-the-art, which may be controversial, but stimulate and promote new ideas, new techniques, and new applications.