Antibacterial Activity of Imported Honey against Methicillin Resistant Staphylococcus aureus (MRSA) Isolated from Infected Wounds

Khadija Muhammad Lawan, Binta Muhammad Aminu, Abdulrazak Muhammad Hussain, A. Yunusa
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Abstract

The use of traditional and herbal medicine to treat infections has been in practice since the origin of mankind, and it was the only option for treatment before the era of antibiotics. This research work was aimed at evaluating the antibacterial activity of imported honey against methicillin resistant Staphylococcus aureus isolated from different wound infections.152Samples from different wounds were obtained from patients with infected wounds attending Murtala Muhammad Specialist Hospital and Muhammadu Abdullahi Wase Specialist Hospital, Kano. The isolates were identified and confirmed using biochemical tests. The antibacterial activity of the honey and minimum inhibitory concentration (MIC) against the isolates were determined using agar well diffusion and two-fold dilution method respectively. The results to detect MRSA revealed its incidence as 24.9%. The result of the antibacterial activity of honey revealed activity at all the concentrations with the diameter of zones of inhibition ranging from 8-34mm.The two imported honey used in the study showed varied bacteriostatic activities, and none of the isolates was resistant to the tested honeys. Therefore, the antibacterial activity of honey even at lower strength justify their efficacy in the treatment of wound infection.
进口蜂蜜对感染伤口耐甲氧西林金黄色葡萄球菌(MRSA)的抑菌活性研究
自人类起源以来,使用传统和草药治疗感染一直在实践中,在抗生素时代之前,这是治疗的唯一选择。本研究旨在评价进口蜂蜜对不同伤口感染源耐甲氧西林金黄色葡萄球菌的抑菌活性。152 .从在卡诺穆尔塔拉·穆罕默德专科医院和穆罕默德·阿卜杜拉希·瓦塞专科医院就诊的伤口感染患者身上采集了不同伤口的样本。采用生化试验对分离株进行鉴定和确认。采用琼脂孔扩散法和二倍稀释法分别测定蜂蜜的抑菌活性和最低抑菌浓度(MIC)。MRSA检测结果显示其发生率为24.9%。结果表明,蜂蜜在不同浓度下均具有抑菌活性,抑菌带直径在8 ~ 34mm之间。研究中使用的两种进口蜂蜜显示出不同的抑菌活性,并且没有一种分离株对所测蜂蜜产生抗性。因此,即使在较低强度下,蜂蜜的抗菌活性也证明了它们在治疗伤口感染方面的功效。
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