Evaluation of Estrogen Receptor-α Gene (T/C) Polymorphism in Patients With Leiomyoma Compared to Healthy Individuals

Abstract

Background: Leiomyoma as one of the most prevalent tumors in women occurs at the same time of reproductive age and its causes are still unknown. Hormone therapy is one of the common treatments for this disease. Estrogen hormone through its receptor, which is called ER α, plays an effective role in the treatment of leiomyoma. Single nucleotide polymorphisms (SNPs) are effective in the diagnosis, treatment, and prognosis of many diseases and tumors. In this regard, the current study investigated the estrogen receptor gene polymorphisms of ER α in women with leiomyoma in Sistan and Baluchestan province, Zahedan and then compared them with healthy individuals. Materials and Methods: Overall, 150 women with leiomyoma were sampled and their DNA was isolated as well. In addition, 150 samples were taken from healthy individuals as the control group. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and PvuII were used to study gene polymorphisms. Results: The results showed a significant relationship between ER α gene polymorphism and leiomyoma. Conclusion: Accordingly, this gene polymorphism can be considered as a marker for prognosis in leiomyoma in the population of Iranian women in Zahedan, Sistan and Baluchestan province.