Distribution of Odontoglossum ringspot virus in apical meristems of infected Cymbidium cultivars

A. Toussaint , D. Dekegel, G. Vanheule
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引用次数: 20

Abstract

The presence of Odontoglossum ringspot virus in the lateral and apical meristem domes of Cymbidium cultivars was revealed by electron microscopy. The analysis of transverse serial ultra-thin sections of apical meristems and of longitudinal serial ultra-thin sections of axillary bud meristems of donor plants, and of apical meristems of plantlets cultured from excised meristems, confirmed the presence of virus in the meristematic regions.

These observations provide an explanation for the difficulties encountered in obtaining virus-free Cymbidium cv plantlets by meristem culture.

牙舌环斑病毒在染病大花蕙兰品种顶端分生组织中的分布
电镜观察发现,大花蕙兰品种的侧边和顶端分生组织穹丘中存在齿舌环斑病毒。对供体植株的根尖分生组织的横向连续超薄切片和腋芽分生组织的纵向连续超薄切片以及从切除的分生组织培养的植株的根尖分生组织进行分析,证实了病毒在分生组织区域的存在。这些观察结果解释了通过分生组织培养获得无病毒大花蕙兰植株的困难。
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