Evaluation of Anticonvulsant Activity of Hydro Ethanolic Extract of Coccinia grandis in Mice

Abstract

Background: Hydro ethanolic extract of Coccinia grandis leaves was assumed to have anticonvulsant activity. Objective: To evaluate the anticonvulsant activity of Coccinia grandis by using some animal models like maximal electric shock model, pentylenetetrazole induced seizures, Isoniazid induced seizures. Materials and Methods: Various equipment and apparatus were used in the present study. Hydroethanolic extract of Coccinia grandis was done by Soxhlet apparatus. Phytochemical investigation of the leaf extract of Coccinia grandis was performed. Anticonvulsant activity was determined by using different animal models like maximal electric shock model, pentylenetetrazole induced seizures, Isoniazid induced seizures. Results: Anticonvulsant activity was determined by using different animal models like Maximal electric shock model, pentylenetetrazole induced seizures & Isoniazid induced model. Percentage of protection, Duration of hind limb tonic extension (HLTE), Latency of onset of convulsions were taken as a indication of anticonvulsant activity. Phenytoin and Diazepam showed significant anticonvulsant activity. In the present study the test samples exhibited significant (P < 0.0001) anticonvulsant activity and antioxidant activity at a dose of 200,400mg/kg. It may due to the presence of alkaloids, Phenols, carbohydrates, glycosides, saponins and flavonoids terpenoids. Conclusion: In present study leaves of Coccinia grandis extract was collected and anticonvulsant activity was examined in a doses of 200mg/kg, 400mg/kg. The results clearly shown that the low dose 200mg/kg of plant extract shown appropriate anticonvulsant activity and the high dose 400mg/kg shown specific anticonvulsant activity when compared to control and standard groups.