Genetic diversity of endangered sandalwood (Osyris lanceolata) populations in Kenya using ISSR molecular markers

Kp Andiego, O. Dangasuk, D. Odee, F. Omondi, D. Otieno, Bk Balozi
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引用次数: 7

Abstract

ABSTRACT Osyris lanceolata is an evergreen, drought tolerant tropical African tree species belonging to the family Santalaceae. It is endangered, owing to overexploitation for its essential oil used in cosmetics and pharmaceutical industries. The study aimed at determining: (1) the genetic diversity; and (2) the population genetic differentiation in seven key O. lanceolata populations, representing its natural distribution in Kenya. Genotype data for ISSR neutral molecular markers were generated for seven populations of O. lanceolata. The percentage of polymorphic loci (P), ranged from 51% (Wundanyi) to 82% (Gwasii), with a mean of 65%. The mean number of effective alleles (Ne) was 1.430, whereas the Shannon Information Index (I) mean was 0.263. Gwasii population was the most genetically diverse followed by Mt Elgon and least was Wundanyi. The coefficient of differentiation Gst was 0.343. Results of analysis of molecular variance (AMOVA) showed that most of the genetic variation (62%) in O. lanceolata resided within populations. Principal coordinate analysis (PCoA) analysis showed that Baringo population located in the Rift Valley was genetically distinct from the rest of the populations. In conclusion, Gwasii, Mt Elgon and Baringo populations should be delineated for in situ conservation, whereas selection for ex situ conservation should target good trees from all the populations.
利用ISSR分子标记分析肯尼亚濒危檀香种群的遗传多样性
杉木(Osyris lanceolata)是一种常绿、耐旱的热带非洲树种,属于杉科。由于过度开采用于化妆品和制药工业的精油,它濒临灭绝。本研究旨在确定:(1)遗传多样性;(2)代表肯尼亚杉木自然分布的7个关键杉木居群遗传分化。对杉木7个居群的ISSR中性分子标记进行了基因型分析。多态性位点(P)的比例从51% (Wundanyi)到82% (Gwasii)不等,平均为65%。有效等位基因数(Ne)均值为1.430,Shannon信息指数(I)均值为0.263。Gwasii种群遗传多样性最高,其次是Mt Elgon种群,Wundanyi种群遗传多样性最低。Gst的分化系数为0.343。分子变异分析(AMOVA)结果表明,大部分遗传变异(62%)发生在群体内。主坐标分析(PCoA)表明,位于东非大裂谷的巴林果居群与其他居群在遗传上存在差异。综上所述,Gwasii、Mt Elgon和Baringo种群应被划定为原地保护,而迁地保护的选择应针对所有种群的优良树木。
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