Molecular identification of Halophilic Microorganisms producing Exo-Halozymes by a culture-dependent approach from the North Sinai Solar saltern

Naglaa Elshafey, A. Elkashef, H. Hamedo, M. Mansour
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引用次数: 3

Abstract

Solar salterns are an ideal model for extreme environments since they contain a halophilic microorganisms that must adapt to these conditions. In North Sinai, these environments have not gotten much attention. However, the focus of our investigation was on the salterns' waters and saline soil. The study of this microflora showed that it had a lot of different shapes, functions, and ways of using energy. The molecular analysis provided us with a phylogenetic relationship that included Aspergillus isolates as well as archaeal isolates from the genera Haloarcula, Haloferex, and Halobacterium, and also bacteria related to Halomonas and Brevibacillus. The majority of isolates expressed hydrolytic enzymes at high salt concentrations. Finally, the North Sinai Solar Saltern is rich in halophilic populations that produce industrial hydrolytic enzymes.
北西奈太阳盐碱地产生外盐酶的嗜盐微生物的分子鉴定
太阳盐沼是极端环境的理想模型,因为它们含有必须适应这些条件的嗜盐微生物。在北西奈,这些环境没有得到太多关注。然而,我们的调查重点是盐碱地的水域和盐碱地。对这种微生物群的研究表明,它有很多不同的形状、功能和使用能量的方式。分子分析为我们提供了一种系统发育关系,包括分离的曲霉,从Haloarcula属,Haloferex属和Halobacterium属分离的古细菌,以及与Halomonas和Brevibacillus相关的细菌。大多数菌株在高盐浓度下表达水解酶。最后,北西奈太阳盐湖富含生产工业水解酶的嗜盐菌群。
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