Ameliorative Effect of Grape Seed Oil on Aluminium Chloride Induced Neurotoxicity on Rats

P. Muralidharan, C. Swetha
{"title":"Ameliorative Effect of Grape Seed Oil on Aluminium Chloride Induced Neurotoxicity on Rats","authors":"P. Muralidharan, C. Swetha","doi":"10.9734/ajbgmb/2023/v14i2310","DOIUrl":null,"url":null,"abstract":"Aims: Neurodegenerative disorder is characterized by progressive loss of structure and function of neurons. Exposure to aluminum causes neurodegenerative disorders like dementia, and Alzheimer's disease (AD). The present study was designed to examine the ameliorative effect of grape seed oil extraction aluminium chloride induced neurotoxicity in Wistar rats. \nMethodology: Wistar rats were administered with aluminium chloride (175 mg/kg. p.o.) for 28 days to generate neurotoxicity model. Attenuation effect of grape seed oil against aluminium chloride toxicity by oral administration adjunctly from day 18. Behavioral and locomotor activity was determined using passive avoidance test, open field test, actophotometer and rota rod test. Biochemical parameter such as acetylcholinesterase (AChE) activity andsuperoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) activity were assessed in brain samplesand histology of brain tissue were assessed on the final day of the experiment. \nResults: Aluminium chloride treatment significantly decreases cognitive function and open field test with a significance decrease in the acetylcholinesterase and antioxidant (SOD, CAT, GR) activity level. Also, the histopathological examination shows significance decrease in the neuronal cell density in hippocampal region. Grape seed oil treated with aluminum chloride neurotoxic groups alleviates all the toxicity induced by the aluminum chloride. Histopathological studies in the hippocampus and cortex of the rat brain also supported that the grape seed oil markedly reduced the toxicity of aluminium chloride. \nConclusion: Supplementation of grape seed oilexhibited with beneficial and neuroprotective role on aluminium chloride induced neurotoxicity in Wistar rat modelby improving the cognitive memory and antioxidant enzyme level.","PeriodicalId":8498,"journal":{"name":"Asian Journal of Biochemistry, Genetics and Molecular Biology","volume":"295 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Asian Journal of Biochemistry, Genetics and Molecular Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.9734/ajbgmb/2023/v14i2310","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Aims: Neurodegenerative disorder is characterized by progressive loss of structure and function of neurons. Exposure to aluminum causes neurodegenerative disorders like dementia, and Alzheimer's disease (AD). The present study was designed to examine the ameliorative effect of grape seed oil extraction aluminium chloride induced neurotoxicity in Wistar rats. Methodology: Wistar rats were administered with aluminium chloride (175 mg/kg. p.o.) for 28 days to generate neurotoxicity model. Attenuation effect of grape seed oil against aluminium chloride toxicity by oral administration adjunctly from day 18. Behavioral and locomotor activity was determined using passive avoidance test, open field test, actophotometer and rota rod test. Biochemical parameter such as acetylcholinesterase (AChE) activity andsuperoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) activity were assessed in brain samplesand histology of brain tissue were assessed on the final day of the experiment. Results: Aluminium chloride treatment significantly decreases cognitive function and open field test with a significance decrease in the acetylcholinesterase and antioxidant (SOD, CAT, GR) activity level. Also, the histopathological examination shows significance decrease in the neuronal cell density in hippocampal region. Grape seed oil treated with aluminum chloride neurotoxic groups alleviates all the toxicity induced by the aluminum chloride. Histopathological studies in the hippocampus and cortex of the rat brain also supported that the grape seed oil markedly reduced the toxicity of aluminium chloride. Conclusion: Supplementation of grape seed oilexhibited with beneficial and neuroprotective role on aluminium chloride induced neurotoxicity in Wistar rat modelby improving the cognitive memory and antioxidant enzyme level.
葡萄籽油对氯化铝所致大鼠神经毒性的改善作用
目的:神经退行性疾病以神经元结构和功能的逐渐丧失为特征。接触铝会导致神经退行性疾病,如痴呆和阿尔茨海默病(AD)。本研究旨在探讨葡萄籽油提取液氯化铝对Wistar大鼠神经毒性的改善作用。方法:Wistar大鼠灌胃氯化铝(175 mg/kg)。P.o .) 28 d生成神经毒性模型。葡萄籽油从第18天起辅助口服对氯化铝毒性的衰减作用。采用被动回避试验、空地试验、温度计和旋转棒试验测定行为和运动活动。在实验的最后一天,测定脑样品的生化指标如乙酰胆碱酯酶(AChE)活性、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽还原酶(GR)活性,并对脑组织进行组织学检查。结果:氯化铝处理显著降低大鼠认知功能和野外试验,显著降低乙酰胆碱酯酶和抗氧化剂(SOD、CAT、GR)活性水平。组织病理学检查显示海马区神经元细胞密度明显降低。经氯化铝神经毒性基团处理的葡萄籽油可减轻氯化铝引起的所有毒性。大鼠海马和大脑皮层的组织病理学研究也支持葡萄籽油显著降低氯化铝的毒性。结论:葡萄籽油通过改善认知记忆和抗氧化酶水平,对氯化铝诱导的Wistar大鼠神经毒性具有有益的神经保护作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信