Cytokine profile in patients with osteoarthritis after SARS-CoV-2 infection

IF 0.7 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
D. Krenytska, L. Kot, T. Halenova, N. Raksha, T. Vovk, O. Savchuk, R. Pellicano, L. Abenavoli, T. Falalyeyeva, L. Ostapchenko
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引用次数: 1

Abstract

BACKGROUND: Severe acute respiratory syndrome-related Coronavirus 2 (SARS-CoV-2) іnfection induces a pro-inflammatory state of an organism with long-term systemic consequences as a result. Systemic inflammation, characterized by a high circulating level of inflammatory cytokines, is a significant factor influencing articular cartilage metabolism in osteoarthritis (OA). This study aimed to determine the levels of pro-inflammatory and anti-inflammatory cytokines in plasma of patients with OA following SARS-CoV-2 infection and to compare them with those of healthy controls. METHODS: The experiment involved patients of the Orthopedic Specialty Clinic aged 46 to 69 diagnosed with knee OA. Among persons with joint pathology a group of convalescent patients from 6-9 months after COVID-19 was identified. The control group involved relatively healthy donors. The plasma levels of pro-inflammatory (IL-1β, IL-6, IL-8, IL-12β, tumor necrosis factor α [TNF-α], interferon-gamma [IFN-γ]) and anti-inflammatory (IL-4 and IL-10) cytokines were determined by enzyme-linked immunosorbent assay. RESULTS: It was established that in patients with OA, as well as after suffering from SARS-CoV-2 infection, an increase in the plasma levels of IL-1β was observed against the background of a decrease in the levels of IL-4, IL-8, IL-10, IL- 12β, TNF-α and IFN-γ, compared to the healthy controls. COVID-19 more significantly influenced the plasma levels of pro-inflammatory cytokines IL-1β and IL-12β. CONCLUSIONS: The results indicate the imbalance of pro- and anti-inflammatory cytokines in the plasma in patients with OA for a long post- COVID. Сhanges in the levels of inflammatory mediators suggest distinct immunoregulatory mechanisms involved in the pathogenesis of both joint pathology and systemic disorders caused by SARS-CoV-2. [ FROM AUTHOR]
SARS-CoV-2感染后骨关节炎患者的细胞因子特征
背景:严重急性呼吸综合征相关冠状病毒2 (SARS-CoV-2)感染可诱导机体的促炎状态,从而导致长期的系统性后果。系统性炎症是影响骨关节炎(OA)患者关节软骨代谢的重要因素,其特征是炎症细胞因子的高循环水平。本研究旨在测定SARS-CoV-2感染后OA患者血浆中促炎和抗炎细胞因子的水平,并与健康对照进行比较。方法:实验对象为46 ~ 69岁骨科专科门诊诊断为膝关节OA的患者。在有关节病理的人群中,一组在新冠肺炎确诊后6-9个月的恢复期患者。对照组是相对健康的捐献者。采用酶联免疫吸附法检测血浆促炎因子(IL-1β、IL-6、IL-8、IL-12β)、肿瘤坏死因子α (TNF-α)、干扰素γ (IFN-γ)和抗炎因子(IL-4、IL-10)水平。结果:与健康对照组相比,OA患者以及SARS-CoV-2感染后,血浆中IL-1β水平升高,而IL-4、IL-8、IL-10、IL- 12β、TNF-α和IFN-γ水平降低。COVID-19对血浆促炎因子IL-1β和IL-12β水平的影响更为显著。结论:新冠肺炎后长期OA患者血浆促炎性因子和抗炎性因子失衡。Сhanges炎症介质水平的变化表明,不同的免疫调节机制参与了SARS-CoV-2引起的关节病理和全身性疾病的发病机制。[源自作者]
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来源期刊
Minerva Biotechnology and Biomolecular Research
Minerva Biotechnology and Biomolecular Research BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
1.50
自引率
30.00%
发文量
22
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