Role of Montelukast in Alleviation of Toxicity Induced by Cadmium in Thyroid Gland of Adult Male Albino Rats. (Histological, Immunohistochemical and Biochemical Study)

the egyptian journal of histology Pub Date : 2020-12-15 DOI:10.21608/ejh.2020.46863.1376

Eman S El-Roghy, R. Yassien

{"title":"Role of Montelukast in Alleviation of Toxicity Induced by Cadmium in Thyroid Gland of Adult Male Albino Rats. (Histological, Immunohistochemical and Biochemical Study)","authors":"Eman S El-Roghy, R. Yassien","doi":"10.21608/ejh.2020.46863.1376","DOIUrl":null,"url":null,"abstract":"Background: Cadmium is a common toxic heavy metal of great concern for environmental pollution and human health. It is extensively used in many industries as radiation screens, rechargeable batteries and pigments. Montelukast is a powerful highly effective anti-inflammatory drug. Recently it shows significant antioxidant properties.Aim of the Study: evaluate the effect of Cadmium on thyroid glands and to assess the possible protective role of MontelukastMaterials and Methods: Forty adult male albino rats were divided into four equal groups received treatment for 6 weeks: (I) (Control group) received distilled water. (II) (Cadmium group)\"3mg/kg\". (III) (Recovery group) kept untreated for another 6 weeks after Cadmium administration with the same dose and period as pervious group. (IV) (Montelukast and Cadmium group) received Montelukast \"10mg/kg\" and Cadmium\"3mg/kg\". At the end, all animals were sacrificed. Thyroid gland samples were obtained for histological and immunohistochemical studies. Blood samples were obtained for hormonal assay. Data was statistically analyzed.Results: Histological examination of thyroid glands of Cadmium treated rat’s showed disorganization and irregularity in the follicles. The follicular cells appeared distorted with pyknotic nuclei and some cell desquamated in follicular lumen. Colloid retraction within the follicles, cellular infiltration around congested blood vessels and marked collagen fibers deposition in connective tissue septae were detected. Significant increase in Caspase-3 immune expression in the follicular cell’s cytoplasm was noticed. High significant decrease of thyroid hormone (T3, T4) level was noticed. These changes were still seen in the recovery group. Co-administration of Montelukast showed obvious protection against the hazardous effects of Cadmium. Conclusion: Cadmium induces marked thyroid tissue damage via, fibrosis and apoptosis. These structural changes can be alleviated by combined treatment with Montelukast.","PeriodicalId":22420,"journal":{"name":"the egyptian journal of histology","volume":"68 1","pages":"0-0"},"PeriodicalIF":0.0000,"publicationDate":"2020-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"the egyptian journal of histology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21608/ejh.2020.46863.1376","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 2

Abstract

Background: Cadmium is a common toxic heavy metal of great concern for environmental pollution and human health. It is extensively used in many industries as radiation screens, rechargeable batteries and pigments. Montelukast is a powerful highly effective anti-inflammatory drug. Recently it shows significant antioxidant properties.Aim of the Study: evaluate the effect of Cadmium on thyroid glands and to assess the possible protective role of MontelukastMaterials and Methods: Forty adult male albino rats were divided into four equal groups received treatment for 6 weeks: (I) (Control group) received distilled water. (II) (Cadmium group)"3mg/kg". (III) (Recovery group) kept untreated for another 6 weeks after Cadmium administration with the same dose and period as pervious group. (IV) (Montelukast and Cadmium group) received Montelukast "10mg/kg" and Cadmium"3mg/kg". At the end, all animals were sacrificed. Thyroid gland samples were obtained for histological and immunohistochemical studies. Blood samples were obtained for hormonal assay. Data was statistically analyzed.Results: Histological examination of thyroid glands of Cadmium treated rat’s showed disorganization and irregularity in the follicles. The follicular cells appeared distorted with pyknotic nuclei and some cell desquamated in follicular lumen. Colloid retraction within the follicles, cellular infiltration around congested blood vessels and marked collagen fibers deposition in connective tissue septae were detected. Significant increase in Caspase-3 immune expression in the follicular cell’s cytoplasm was noticed. High significant decrease of thyroid hormone (T3, T4) level was noticed. These changes were still seen in the recovery group. Co-administration of Montelukast showed obvious protection against the hazardous effects of Cadmium. Conclusion: Cadmium induces marked thyroid tissue damage via, fibrosis and apoptosis. These structural changes can be alleviated by combined treatment with Montelukast.

查看原文本刊更多论文

孟鲁司特减轻镉对成年雄性白化大鼠甲状腺毒性的作用。(组织学、免疫组化和生化研究)

背景:镉是一种常见的有毒重金属，对环境污染和人类健康有着重要影响。它广泛应用于许多行业，如防辐射屏，可充电电池和颜料。孟鲁司特是一种强力高效的消炎药。最近它显示出显著的抗氧化性能。研究目的:评价镉对甲状腺的影响及孟鲁司特可能的保护作用。材料与方法:40只成年雄性白化大鼠分为4组，每组治疗6周:(1)(对照组)给予蒸馏水。(二)(镉组)“3mg/kg”。(III)(恢复组)镉给药后继续治疗6周，剂量和周期与前一组相同。(四)孟鲁司特加镉组:孟鲁司特10mg/kg，镉3mg/kg。最后，所有的动物都被牺牲了。甲状腺标本用于组织学和免疫组织化学研究。取血样作激素测定。对数据进行统计学分析。结果:镉处理大鼠甲状腺组织组织学检查显示，甲状腺滤泡紊乱、不规则。滤泡细胞变形，细胞核固缩，滤泡腔内部分细胞脱落。滤泡内胶质缩回，充血血管周围细胞浸润，结缔组织隔胶原纤维沉积明显。滤泡细胞细胞质中Caspase-3免疫表达明显升高。甲状腺激素(T3、T4)水平明显降低。这些变化在恢复组中仍然可见。同时服用孟鲁司特对镉的有害影响有明显的保护作用。结论:镉对甲状腺组织具有明显的损伤、纤维化和凋亡作用。这些结构变化可通过联合孟鲁司特治疗得到缓解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

the egyptian journal of histology

自引率

0.00%

发文量