Molecular Divergence of Staphylococcus Aureus Isolated from Pork Byproducts Based on Coagulase Gene Polymorphism and The Presence of Enterotoxigenic genes

Abstract

Background: Pork by-products are appeared to be a source of consumer coagulase positive S. aureus strains food poisoning. Material and Methods: The genetic lineage of coagulase positive Staphylococcus aureus (coa) recovered from 20 imported and 30 locally manufactured pork byproducts in Cairo was checked using PCR, and restriction fragment length polymorphism analysis (RFLP). Results: A total number of 27 S. aureus were isolated (54%), the coa gene was identified in 17 out of that isolates (62.9%) with molecular sizes ranging from 195 bp to 1196bp. The coa RFLP analysis using BsuRI revealed 4 different band patterns related to the source of isolates. The detection of 7 enterotoxigenic strains out of the 27 S. aureus isolates suggested the public health importance. Conclusion: There were different polymorphism patterns between local and imported isolates with the detection of enterotoxigenic genes represent a public health concern.