Influence of reduced amounts of sulfoquinovosyl diacylglycerol on the thylakoid membranes of the diatom Thalassiosira pseudonana.

Abstract

Diatom thylakoids contain much higher amounts of sulfoquinovosyl diacylglycerol (SQDG) than vascular plants and the hypothesis was brought forward that this relates to their special thylakoid structure. To test this hypothesis we created knock-down mutants in Thalassiosira pseudonana that exhibited a decreased SQDG content per cell. Surprisingly, the ratio between the different lipid classes did not change, pointing to strict regulation of thylakoid lipid composition. The antenna proteins, fucoxanthin-chlorophyll proteins (FCP), were reduced and photosystem (PS) I compared to PSII was increased as judged from absorbance spectra. CD spectroscopy indicated a tighter packing of chromophores. The reduction in FCP might help to avoid diametral changes in excitation energy transfer. In contrast, the increase in PSI in the mutants might counteract the diminishment of the usually huge PSI antenna. No changes in thylakoid structure were observed since the stoichiometry between different lipid classes seems to be carefully balanced.