Anti-haemolytic and cytogenotoxic potential of aqueous leaf extract of Annona muricata (L.) and its bio-fabricated silver nanoparticles

IF 2.1 4区生物学 Q2 Agricultural and Biological Sciences

Caryologia Pub Date : 2022-07-07 DOI:10.36253/caryologia-1353

Badmus Jelili Abiodun, S.A. Oyemomi, J. Fatoki, T. Yekeen, O. Adedosu, P. Adegbola, M. A. Azeez, E. Adebayo, A. Lateef

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引用次数: 2

Abstract

Nanotechnology is widely gaining worldwide application in biology and medicine because of its proven efficacy. Annona muricata contains bioactive phytochemicals with an inherent ability to bio-fabricate metal ions nanoparticles (NPs). Annona muricata aqueous leaf extract and its green bio-fabricated silver nanoparticles were evaluated on red blood cells (RBC) for anti-haemolytic activity and cytogenotoxicity on Allium cepa cells. The effects of A. muricata extract (Am-E) and its biofabricated silver nanoparticles (Am-AgNPs) were observed at 0.7, 7.0 and 70.0 µg/ml on H2O2-induced haemolysis in RBC and cyclophosphamide-induced cytogenotoxicity on A. cepa cells. Results showed significant and concentration dependent anti-haemolytic activity of Am-E relative to Am-AgNPs. Significant (P<0.05) reduction of mitotic index was observed in the groups treated with Am-AgNPs compared with Am-E, which indicates cytotoxic effect of the nanoparticles. The Am-E protected A. cepa meristem root cells from cyclophosphamide-induced mitotic repression better than Am-AgNPs. Different degree of chromosomal abnormalities such as chromosome-bridge, sticky chromosome, and c-mitosis were observed in all the treatment groups with chromosome-bridge and sticky chromosome being prominent. This study revealed stronger anti-haemolytic efficacy of Am-E at higher concentrations compared with Am-AgNPs. Chromosomal abnormalities observed in this study suggest greater chromosomal instability as influenced by the nanoparticles compared with the extract on onion cells. The protective effect of the extract against cyclophosphamide-induced chromosomal aberrations may be an indication of its potential as an anti-genotoxic agent.

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番荔枝叶水提物及其制备银纳米颗粒的抗溶血和细胞毒性研究

纳米技术由于其已被证实的有效性，在生物和医学领域得到了广泛的应用。番荔枝含有具有生物活性的植物化学物质，具有生物制造金属离子纳米颗粒(NPs)的固有能力。研究了番荔枝叶水提物及其绿色生物合成纳米银在红细胞(RBC)上的抗溶血活性和对洋葱细胞的细胞遗传毒性。研究了0.7、7.0和70.0µg/ml浓度的野牡丹提取物(Am-E)及其制备银纳米粒子(Am-AgNPs)对h2o2诱导的红细胞溶血和环磷酰胺诱导的野牡丹细胞遗传毒性的影响。结果显示Am-E相对于Am-AgNPs具有显著的浓度依赖性抗溶血活性。与Am-E相比，Am-AgNPs组有丝分裂指数显著降低(P<0.05)，表明纳米颗粒具有细胞毒性作用。Am-E比Am-AgNPs更能保护cepa分生组织根细胞免受环磷酰胺诱导的有丝分裂抑制。各治疗组均有不同程度的染色体异常，如染色体桥、粘染色体、c-有丝分裂等，且以染色体桥、粘染色体突出。本研究表明，与Am-AgNPs相比，高浓度Am-E的抗溶血作用更强。本研究中观察到的染色体异常表明，与洋葱细胞上的提取物相比，纳米颗粒对染色体不稳定性的影响更大。提取物对环磷酰胺诱导的染色体畸变的保护作用可能表明其作为抗遗传毒性药物的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Caryologia 生物-遗传学

CiteScore

1.60

自引率

23.80%

发文量

审稿时长

12 months

期刊介绍： Caryologia is devoted to the publication of original papers, and occasionally of reviews, about plant, animal and human karyological, cytological, cytogenetic, embryological and ultrastructural studies. Articles about the structure, the organization and the biological events relating to DNA and chromatin organization in eukaryotic cells are considered. Caryologia has a strong tradition in plant and animal cytosystematics and in cytotoxicology. Bioinformatics articles may be considered, but only if they have an emphasis on the relationship between the nucleus and cytoplasm and/or the structural organization of the eukaryotic cell.