Sequence feature and expression profile of a tumor suppressor QM protein gene from hard clam Meretrix meretrix

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2019-10-14 DOI:10.25431/1824-307X/ISJ.V0I0.164-172

X. Cui, J. W. Cheng, Yan Wang, B. Zhou

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引用次数: 1

Abstract

The present study describes the molecular characterization and transcriptional features of a tumor suppressor QM protein gene, MmQM, in Meretrix meretrix. The full-length cDNA (819 bp) of MmQM consists of a 657 bp opening reading frame (ORF) encoding a 218 amino acid protein with a calculated molecular mass of 25.3 kDa and theoretical isoelectric point of 10.2. A ribosomal protein L10 signature, an SH3-binding motif, an antibiotic binding site, an amidation site and two protein kinase C phosphorylation sites were revealed from the MmQM sequence. Phylogenetic analysis showed that MmQM is clustered with previously identified mollusk QM proteins. MmQM mRNA transcripts were detectable in all of the examined tissues in a constitutive manner, and were significantly different from each other. After bacterial stimulation, the mRNA transcripts of MmQM in the hepatopancreas significantly increased. Hence, we conclude that MmQM could respond to pathogenic infections and it might play an important role in the innate immunity against microorganisms in the clam M. meretrix.

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硬蛤肿瘤抑制基因QM的序列特征及表达谱分析

本研究描述了Meretrix Meretrix中肿瘤抑制因子QM蛋白基因MmQM的分子特征和转录特征。MmQM全长cDNA (819 bp)由657 bp的开放阅读框(ORF)组成，编码218个氨基酸的蛋白，计算分子量为25.3 kDa，理论等电点为10.2。从MmQM序列中发现了一个核糖体蛋白L10特征、一个sh3结合基序、一个抗生素结合位点、一个酰胺化位点和两个蛋白激酶C磷酸化位点。系统发育分析表明，MmQM与先前鉴定的软体动物QM蛋白聚类。MmQM mRNA转录本在所有检测组织中均可检测到，并且彼此之间存在显著差异。细菌刺激后，肝胰脏中MmQM mRNA转录量显著增加。因此，我们认为MmQM对致病性感染有应答作用，可能在蛤蚌对微生物的先天免疫中起重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.