{"title":"Production of Lavender (Lavandula Angustifolia) Plants from Somatic Embryos Developed from its Seedlings Leaf Callus","authors":"Azza Al-Tai, A. Mohammed","doi":"10.33899/rjs.2022.176073","DOIUrl":null,"url":null,"abstract":"The current study succeeded in producing the lavender ( Lavandula angustifolia ) plant from somatic embryos induced by the leaves callus of its seedlings, The results of soaking its dormant seeds in a solution of gibberellic acid at concentrations (0, 0.1, 0.25, 0.5, 1.0) gm l -1 for 24 hours showed the superiority of the concentration 0.5 g L -1 in stimulation the germination rate that reached 75% after 6 days comparing with other treatments and control that had 20% germination after 13 days. This study was able to initiated callus from seedling leaves when were placed on MS solid medium supplemented with by added 3.0 mg L -1 NAA and 10 mg L -1 BA after 20 days, Initiated callus was characterized by its yellowish-green color and friable texture, and when its masses were removed and placed on the same media, it produced well-growth cultures that were perpetuated every 15 days. After the second sub-culturing, the first stages of somatic embryos appeared, the spherical stage, which later developed into the heart,then torpedo and cotyledon stages and produced when placed individually on MSO medium, Intact lavender plants after 30 days were characterized by their good growth and density of leaves.","PeriodicalId":20803,"journal":{"name":"Rafidain journal of science","volume":"38 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Rafidain journal of science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.33899/rjs.2022.176073","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
The current study succeeded in producing the lavender ( Lavandula angustifolia ) plant from somatic embryos induced by the leaves callus of its seedlings, The results of soaking its dormant seeds in a solution of gibberellic acid at concentrations (0, 0.1, 0.25, 0.5, 1.0) gm l -1 for 24 hours showed the superiority of the concentration 0.5 g L -1 in stimulation the germination rate that reached 75% after 6 days comparing with other treatments and control that had 20% germination after 13 days. This study was able to initiated callus from seedling leaves when were placed on MS solid medium supplemented with by added 3.0 mg L -1 NAA and 10 mg L -1 BA after 20 days, Initiated callus was characterized by its yellowish-green color and friable texture, and when its masses were removed and placed on the same media, it produced well-growth cultures that were perpetuated every 15 days. After the second sub-culturing, the first stages of somatic embryos appeared, the spherical stage, which later developed into the heart,then torpedo and cotyledon stages and produced when placed individually on MSO medium, Intact lavender plants after 30 days were characterized by their good growth and density of leaves.
当前的研究成功地生产了薰衣草(薰衣草花angustifolia)从体细胞胚胎诱导植物幼苗的叶片愈伤组织,结果其休眠种子浸泡在赤霉酸的溶液浓度(0,0.1,0.25,0.5,1.0)gm l 1 24小时显示的优越性浓度0.5 g l 1刺激萌发率达到75%后6天比较与其他治疗和控制了20%发芽后13天。本研究将幼苗叶片置于添加3.0 mg L -1 NAA和10 mg L -1 BA的MS固体培养基上,培养20天后即可形成愈伤组织,愈伤组织呈黄绿色,质地易碎,将其块状去除,置于同一培养基上,每15天培养一次。第二次继代培养后,在MSO培养基上分别培养出第一期体细胞胚、球形胚、心胚、鱼雷胚和子叶胚。培养30 d后,完整植株生长良好,叶片密度高。