Cytotoxicity of metals toward rainbow trout R1 cell line

H. Segner, D. Lenz, W. Hanke, G. Schüürmann
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引用次数: 38

Abstract

Cultured fish cells are of potential use in the initial screening of the adverse effects of aquatic pollutants. In the present study, R1 cells, a fibroblast-like cell line derived from liver tissue of rainbow trout (Oncorhynchus mykiss), were exposed to 13 metal salts. Cytotoxicity was assessed by the neutral red uptake inhibition test. The rank order of cytotoxicity for the cationic metals was silver > mercury > cadmium > zinc > copper > nickel > lead; for the anionic metal complexes it was arsenite > dichromate > chromate > arsenate > selenite > permanganate > selenate. Metal cytotoxicity toward R1 cells showed close correlation with the cytotoxicity values of metals toward the BF-2 cell line from bluegill sunfish (r = 0.98). For the divalent cations, cytotoxicity correlated well with the physicochemical softness parameter σp (r = 0.93). Correlation coefficients between the in vitro toxicity of cationic metals and their in vivo LC50 data varied for different fish species, with r values from r = 0.64 to r = 0.92. The in vitro cytotoxicity of anionic metal complexes was not related with their acute in vivo toxicity. © 1994 by John Wiley & Sons, Inc..
金属对虹鳟鱼R1细胞系的细胞毒性
培养的鱼细胞在初步筛选水生污染物的不良影响方面具有潜在的用途。本研究将虹鳟鱼肝组织的成纤维细胞样细胞系R1细胞暴露于13种金属盐中。用中性红摄取抑制试验评价细胞毒性。阳离子金属的细胞毒性排序为银>汞>镉>锌>铜>镍>铅;阴离子金属配合物为亚砷酸盐>重铬酸盐>铬酸盐>砷酸盐>亚硒酸盐>高锰酸盐>硒酸盐。金属对R1细胞的细胞毒性与金属对蓝鳃翻车鱼BF-2细胞系的细胞毒性值密切相关(r = 0.98)。对于二价阳离子,细胞毒性与理化柔软度参数σp (r = 0.93)有良好的相关性。不同鱼种阳离子金属的体外毒性与其体内LC50数据的相关系数不同,r值为r = 0.64 ~ r = 0.92。阴离子金属配合物的体外细胞毒性与体内急性毒性无关。©1994 by John Wiley & Sons, Inc.。
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