Isolation and Purification of Fibrino(geno)lytic Enzymes from Agkistrodon Blomhoffi Usuriensis Venom

Abstract

This paper describes the purification of fibrino(geno)lytic enzymes from Agkistrodon blomhoffii ussuriensis venom in three chromatographic stages. Two single-stranded enzymes with molecular weight of 30 and 37 kDa were separated. Both toxins were purified using affinity, hydrophobic interaction and ion-exchange chromatography and were identified as metaloproteinase and serine protease respectively