A test battery of bacterial toxicity assays and comparison with LD50 values

P. Lenz, R. Süssmuth, E. Seibel
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引用次数: 10

Abstract

The toxicity of some mycotoxins, lactones and dicarboxylic anhydrides, insecticides, herbicides, and fungicides can be detected by means of bacterial assays. In addition, the order of magnitude of the toxicity can also be evaluated. Fifty-eight chemicals as well as extracts of peanuts, apple juices, and grains have been investigated in a test battery of three different kinds of assays. In cup plate diffusion assays a minimal amount of approximately 0. 1 mg/mL of mycotoxin may be detected by means of growth inhibition assays with Bacillus thuringiensis on one hand, and pigment synthesis inhibition assays with mutants of Serratia marcescens on the other hand. Swarming inhibition assays with the motile strains Azospirillum brasilense and Proteus mirabilis show the same sensitivity. Comparisons of our test battery with other bioassays, as well as with mammalian toxicity tests (LD50 values), reveal correlations between these kinds of assays in regard to the test substances.
一种细菌毒性测定和LD50值比较的试验电池
一些真菌毒素、内酯和二羧酸酐、杀虫剂、除草剂和杀菌剂的毒性可以通过细菌试验来检测。此外,还可以评估毒性的数量级。58种化学物质以及花生、苹果汁和谷物的提取物在三种不同的试验中进行了研究。在杯盘扩散试验中,最小量约为0。通过苏云金芽孢杆菌生长抑制试验和粘质沙雷氏菌突变体色素合成抑制试验,可以检测出1 mg/mL的霉菌毒素。对运动菌株巴西偶氮螺旋菌和变形杆菌的群体抑制试验显示出相同的敏感性。将我们的测试电池与其他生物测定法以及哺乳动物毒性试验(LD50值)进行比较,揭示了这些类型的测定法与测试物质之间的相关性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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