Genotypic screening of tomatos AREB 1 gene for drought tolerance and computational protein structure prediction

Abstract

In this investigation, the coding sequence of the drought-stress inducible gene AREB1 in tomato derived from cDNA indicated 100% identity with the reference gene in the NCBI PlantEnsembl database. The protein structure of the AREB1 sequence derived from polymerase chain reaction from tomato DNA template was done using ExPASy and its protein parameter tools ProtParam. The structures of AREB1 protein showed a MolProbity score of 1.49. Multiple sequence alignment of AREB1 gene from 20 tomato genotypes revealed a phylogenetic tree with five clusters, each with the same evolutionary trend. The nucleotide sequence analysis showed higher similarities among the selected tomato genotypes. This indicated the conserved nature of the gene among the genotypes.   Key words: Tomato, drought, resistant, AREB1gene, AREB1protein.