Comparing the Seizure-induced Impairment of Short-term Plasticity in Dorsal and Ventral Hippocampus in Kindled Mice.

Nahid Roohi, Mahboubeh Ahmadi, Yaghoub Fathollahi, Amir Shojaei, Javad Mirnajafi-Zadeh
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Abstract

Introduction: The differences among dorsal and ventral hippocampal neural circuits affect synaptic plasticity. In this study, we aim to compare the occurrence of short-term plasticity in the field excitatory postsynaptic potential (fEPSP) in dorsal and ventral hippocampal CA1 areas following kindled seizures.

Methods: Animals (male C57 B6/J mice, 12 weeks of age) were kindled by intraperitoneal injections of pentylenetetrazole (PTZ), and fEPSPs were recorded from dorsal and ventral hippocampal slices. Short-term plasticity was evaluated by measuring fEPSP-slope and fEPSP-area following paired-pulse stimulation delivered at three inter-pulse intervals (20, 80, and 160 ms).

Results: In control slices, the fEPSP-slope was greater in the ventral hippocampus compared to the dorsal hippocampus, but there was no difference in the fEPSP-area between the two regions. In hippocampal slices, the fEPSP-slope was similar in the dorsal and ventral regions, but the fEPSP-area was greater in the ventral region compared to the dorsal region. In addition, the fEPSP-area was greater in the kindled group than in the control group only in the ventral hippocampus. PTZ-induced kindled slices showed impaired short-term facilitation, and the paired-pulse index decreased only in the dorsal hippocampus. Kindling had no significant effect on the paired-pulse ratio in the ventral hippocampus.

Conclusion: The seizure occurrence affects the neural activity of the hippocampus in a region-dependent manner. Although kindling increases the fEPSP area in the ventral hippocampus of mice, kindling-induced changes in short-term synaptic plasticity are significant only in the dorsal hippocampus. The difference in the responses of dorsal and ventral poles should be considered in future studies.

癫痫致点燃小鼠海马背侧和腹侧短期可塑性损伤的比较。
海马背侧和腹侧神经回路的差异影响突触的可塑性。在这项研究中,我们的目的是比较点燃癫痫发作后海马背侧和腹侧CA1区场兴奋性突触后电位(fEPSP)的短期可塑性的发生。方法:动物(雄性C57 B6/J小鼠,12周龄)腹腔注射戊四唑(PTZ)点燃,海马背侧和腹侧切片记录fEPSPs。在三个脉冲间隔(20、80和160 ms)进行配对脉冲刺激后,通过测量fepsp斜率和fepsp面积来评估短期可塑性。结果:在对照切片中,海马腹侧区fepsp斜率大于海马背侧区,但两个区域的fepsp面积没有差异。在海马切片中,背侧和腹侧区域的fepsp斜率相似,但腹侧区域的fepsp面积大于背侧区域。此外,点燃组仅海马腹侧区fepsp面积大于对照组。ptz诱导的点燃切片显示短期促进功能受损,配对脉冲指数仅在海马背侧下降。点燃对海马腹侧成对脉冲比无显著影响。结论:癫痫发作对海马神经活动的影响具有区域依赖性。虽然点燃增加了小鼠海马腹侧的fEPSP区域,但点燃引起的短期突触可塑性变化仅在海马背侧显著。在未来的研究中应考虑背极和腹极反应的差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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