Water extrusion in isolated subcellular fractions

Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects Pub Date : 1964-12-02 DOI:10.1016/0926-6542(64)90027-7

Renzo Rendi

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引用次数: 14

Abstract

Suspensions of phospholipids, prepared from rat-liver mitochondria or from soya bean, extrude water when serum albumin and a Mg²⁺-ethylenediaminetetraacetate complex is addes. None of many other chelators tested can replace ethylenediaminetetraacetate. The pH optimum is 7.1 for the soya-bean phosphatides and 8.5 for those prepared from mitochondira. Various characteristics of the reaction are similar to the one observed in dialysed rat-liver mitochondira, although in this latter system serum albumin is not required. While in the dialyzed-mitochondira system Ca²⁺ can be replaced by Mg²⁺, with the isolated phospholipid Mg²⁺ is unable to replace Ca². When dialyzed mitochondria are treate with proteolytic enzymes, the requirement for serum albumin is observed and one also finds that Mg²⁺ is now unable to replace Ca²⁺. Little decrease of water-extrusion reaction ibn dialyzed mitochondria occurs after neutral-lipid extraction, while a large decrease is seen after phospholipid removal.

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水挤压分离亚细胞组分

当加入血清白蛋白和Mg2+-乙二胺四乙酸络合物时，从大鼠肝线粒体或大豆制备的磷脂悬浮液会挤出水。测试的许多其他螯合剂都不能取代乙二胺四乙酸酯。大豆磷脂的最适pH为7.1，线粒体磷脂的最适pH为8.5。该反应的各种特征与在透析的大鼠肝线粒体中观察到的相似，尽管在后一种系统中不需要血清白蛋白。而在透析线粒体系统中，Ca2+可以被Mg2+取代，而分离的磷脂Mg2+不能取代Ca2。当透析的线粒体用蛋白水解酶处理时，观察到对血清白蛋白的需求，并且还发现Mg2+现在不能取代Ca2+。中性脂提取后，透析线粒体的水挤压反应减少较少，而磷脂去除后，水挤压反应减少较多。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biochimica et Biophysica Acta (BBA) - Specialized Section on Lipids and Related Subjects

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