Raman Microimaging Using a Novel Multifiber-Based Device: A Feasibility Study on Pharmaceutical Tablets

Abstract

Raman microimaging is a potential analytical technique in health field and presents many possible pharmaceutical applications. In this study, we tested a micrometer spatial resolution probe coupled to a portable Raman imager via an indexed multifiber bundle. At the level of the probe, the fibers were arranged in a circular geometry in order to fit to the pupil of an objective. The imaging potential of this Raman system was assessed on pharmaceutical-like pellets. We showed that this setup permits to record, nearly in real time, Raman images with a micrometer resolution. The collected images revealed a marked heterogeneity in chemicals distribution. Further investigations will be led on cells and biological tissues to evaluate the potential of this Raman imaging device for biomedical applications.