Effects of Osthole on Protein Expression of CYP7A1, DGAT2 and PPARalpha/gamma in Fat Milk-Induced Fatty Liver Rats

2008 2nd International Conference on Bioinformatics and Biomedical Engineering Pub Date : 2008-05-16 DOI:10.1109/ICBBE.2008.63

Zhang Yan, Xie Mei-lin, Xue Jie, Gu Zhen-lun

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Abstract

To determine the effect of osthole on protein expression of PPARalpha/gamma and related target molecules such as CYP7A and DGAT in the liver of hyperlipidemic fatty liver (HFL) rats, and to investigate the possible mechanism of treating HFL. HFL rat model was established by feeding the Sprague-Dawley rats with fat milk for 6 weeks. The experimental rats were then treated with 5-20 mg/kg osthole for 6 weeks. The proteins expression of PPARalpha/gamma, CYP7A1, and DGAT2 in rat liver was measured by western blot analyses. After administration of osthole, expression of PPARalpha and PPARgamma proteins in HFL rat liver was increased by 49% ~ 133% and 19% ~ 113% in the osthole groups as compared with model group, respectively (P<0.05 or P<0.01). The related target proteins were increased by 85% ~ 147% for CYP7A1 and decreased by 35% ~ 62% for DGAT2 by the osthole-treatment, respectively (P<0.01). Osthole possessed therapeutic effects on fat milk-induced fatty liver in rats, and the mechanism might be attributed to regulating the protein expression of CYP7A1 and DGAT2 via increasing PPARalpha/gamma.

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蛇床子素对脂乳性脂肪肝大鼠CYP7A1、DGAT2和ppar / γ蛋白表达的影响

目的:探讨蛇床子素对高脂血性脂肪肝(HFL)大鼠肝脏中ppar / γ及相关靶分子CYP7A、DGAT蛋白表达的影响，探讨其治疗HFL的可能机制。采用脂乳喂养大鼠6周，建立HFL大鼠模型。以5 ~ 20 mg/kg蛇床子素给药6周。western blot法检测大鼠肝脏中ppar / γ、CYP7A1、DGAT2蛋白的表达。给药后，与模型组比较，蛇床子组HFL大鼠肝脏中pparα和pparγ蛋白表达分别升高49% ~ 133%和19% ~ 113% (P<0.05或P<0.01)。经蛇床子酚处理后，CYP7A1相关靶蛋白水平升高85% ~ 147%，DGAT2相关靶蛋白水平降低35% ~ 62% (P<0.01)。蛇床子素对脂肪乳诱导的大鼠脂肪肝具有治疗作用，其机制可能是通过增加ppar - α / γ来调节CYP7A1和DGAT2蛋白的表达。

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2008 2nd International Conference on Bioinformatics and Biomedical Engineering

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