PHYSIOCHEMICAL ANALYSIS AND HEAVY METAL TOLERANCE POTENTIAL OF BACTERIA FROM TEXTILE EFFLUENTS

A.M. Afzal
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Abstract

Heavy metal tolerant bacterial species were isolated from the textile effluents collected from Faisalabad, Pakistan. Thirty samples were subjected to heavy metal analysis. Nickel and Cobalt were selected for further study on the basis of their predominant concentration in all samples. Thirteen samples were screened out as positive for Ni tolerant bacteria and 03 of them had typical characteristics which tolerated Ni up to 08 mM. These species were identified as AMIC1 (Klebsiella spp.), AMIC2 (Bacillus spp.) and AMIC3 (Bacillus spp.). These indigenous strains exhibited pronounced tolerance to Ni and Co and showed multi metal resistance (MMR) potential. Bacteria isolated from sample SarDP2 were able to tolerate Co up to 06mM and exhibited MMR to Ni, Co and Cr (1:1:1) up to 5.5mM. Isolates from RgrDP3 were able to tolerate Co up to 07mM and showed MMR to Ni, Co and Cr (1:1:1) up to 4.5mM. Similarly, isolates from SarDP5 were able to tolerate Co up to 6.5mM and exhibited MMR to Ni, Co and Cr (1:1:1) up to 4.5mM. It was concluded that these species had significant heavy metal tolerance potential and may be used for the development of effective bioremediation agents to detoxify textile effluents at industrial surroundings within natural environments.
纺织废水中细菌的理化分析及重金属耐受性
从巴基斯坦费萨拉巴德收集的纺织废水中分离出耐重金属细菌。对30个样品进行了重金属分析。根据镍和钴在所有样品中的优势浓度,选择镍和钴进行进一步研究。筛选出耐镍菌13份,其中耐镍菌03份具有典型耐镍特征,耐镍最高可达08 mM,分别鉴定为AMIC1(克雷伯氏菌属)、AMIC2(芽孢杆菌属)和AMIC3(芽孢杆菌属)。这些本地菌株对镍和钴表现出明显的耐受性,并显示出多金属抗性(MMR)潜力。从SarDP2样品中分离的细菌能够耐受Co高达06mM,对Ni, Co和Cr(1:1:1)的MMR高达5.5mM。RgrDP3对Co的耐受性可达07mM,对Ni、Co和Cr(1:1:1)的耐受性可达4.5mM。同样,SarDP5的分离株能够耐受6.5mM以下的Co,并对4.5mM以下的Ni, Co和Cr(1:1:1)表现出MMR。结果表明,这些物种具有较强的重金属耐受性,可用于开发有效的生物修复剂,在自然环境中对工业环境中的纺织废水进行脱毒处理。
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