Butylated hydroxytoluene (bht) improves the post-thaw semen quality in low-dose sperm cryopreservation in murrah buffalo bull.

IF 1 4区生物学 Q3 BIOLOGY

Cryo letters Pub Date : 2023-01-01 DOI:10.54680/fr23110110612

D. Nain, T. Mohanty, R. Dewry, M. Bhakat, S. Nath, V. Gupta, M. A. Parray

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引用次数: 1

Abstract

BACKGROUND Cryopreservation is an important technique for the long-term storage of semen for artificial insemination (AI). Buffalo spermatozoa are sensitive to cryopreservation procedures because of the presence of a high amount of polyunsaturated fatty acids in the plasma membrane. OBJECTIVE To study the effect of different concentrations of BHT on the quality of Murrah buffalo bull semen for low-dose cryopreservation. MATERIAL AND METHODS Semen was collected from four high fertile Murrah buffalo bulls (6 ejaculates each) using an artificial vagina. A total of 24 ejaculates were collected from each bull twice a week using an artificial vagina. Every sample was split into four parts: Control without additives; and three treatments with BHT at 0.5 mM, 1 mM or 2 mM. Semen was cryopreserved at low-dose sperm cryopreservation of 20, 15, 10 and 5 million sperm per aliquot after supplementation of BHT. Semen samples were evaluated for fresh, pre-freeze and post-thaw stages. RESULTS There was a significant increase (p<0.05) in sperm quality parameters, such as progressive motility (%), viability (%), HOST response (%), acrosome integrity (%) and post-thaw motility, with the addition of 0.5-1 mM BHT. CONCLUSION The addition of BHT in Murrah buffalo semen improves the low dose cryopreservation quality in a dose-dependent manner. doi.org/10.54680/fr23110110612.

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丁基羟基甲苯(bht)可改善默拉水牛低剂量精子冷冻保存的解冻后精液质量。

低温保存是长期保存人工授精精液的一项重要技术。水牛精子对低温保存程序很敏感，因为在质膜中存在大量的多不饱和脂肪酸。目的研究不同浓度BHT对默拉水牛精液低剂量冷冻保存质量的影响。材料与方法采用人工阴道采集4头高生殖力默拉水牛(每头6次射精)精液。每只公牛每周两次使用人工阴道共采集24次射精。每个样品分为四部分:不添加添加剂的对照组;分别以0.5 mM、1 mM、2 mM三种浓度的BHT处理精液。分别在添加BHT后，分别以20、15、10、500万个精子的低剂量冷冻保存精液。精液样本在新鲜、冷冻前和解冻后阶段进行评估。结果添加0.5-1 mM BHT可显著提高精子进行性活力(%)、活力(%)、HOST反应(%)、顶体完整性(%)和解冻后活力(p<0.05)。结论Murrah水牛精液中添加BHT可提高低剂量低温保存质量，且呈剂量依赖性。doi.org/10.54680/fr23110110612。

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来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.