Sequence Analysis of rDNA ITS of Clinical Fusarium Species

Abstract

The study on molecular structure of Fusarium proliferatum clinically isolated can provide the basis for accurate diagnosis and treatment of F. proliferatum infection. The rDNA ITS gene of 6 strains that had been identified as Fusarium proliferatum based on morphological were cloned and sequenced. The molecule phylogenetic tree was constructed based on rDNA ITS sequences, and the 6 genus were divided into two groups. Group was F.proliferatum, including strains of CSUFT201, CSUFT204, CSUFT205. Group was F. moniliforme, including CSUFT202,CSUFT203,CSUFT206. The results of this study showed that 3 strains of F. moniliforme were confirmed out of the 6 strains with the molecular methods. Keywords-Fusarium; ITS gene; clone; phylogenetic; sequence analysis